What do you think of this CIP

Pierre Lindenbaum lindenb at jouy.inra.fr
Thu Jan 15 10:08:54 EST 1998


Hello,
Well, it seems to me that it is easer to CIP a vector when there is a 3' 
overhang rather than a blunt end , doesn't it ?

So, if I want a blunt-ends CIPed plasmid, wouldn't it be easer to digest
with an enzyme releasing a 3' overhang (Ex: EcoRI), CIP it, and fill it
with the klenow DNA pol ? No ? 




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