Electrotransfer of high MW proteins

PbZep pbzep at hotmail.com
Mon Jan 19 16:49:06 EST 1998


Hi, I also tried this for a large protein 150kda, could not get it to
work. Only thing that was effective was o/n in tank at 4C at 20 volts.
High volts and less time did not work well hope this help.  My
experience with semi-dry  transfer is that it is only effective for
proteins under 70kda. gregg  email invalid



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