william at neuro.usc.edu
Wed Jan 21 16:55:42 EST 1998
I have a 600bp DNA fragment which I cut out of a plasmid, purified from agarose
gel with Gene Clean and labeled with 32P using random prime method. I then
used the probe to screen a lamba ZAP cDNA library (Stratagene) and
isolated several clones. I isolated the clones and cut the inserts out for
a southern blot. I then hybridized the blot with the same 600bp probe. To my
surprise, I don't see any signals on the inserts. Instead, I see signals
representing the 3 kb Bluescript vector and the bands in the 1 kb ladder!
Anyone can think of an explanation?
William Sun, Ph.D Phone: (619) 622-3930
Metabasis Therapeutics, Inc. FAX: (619) 622-5545
9390 Towne Centre Drive Pager: (310) 243-9878
San Diego, CA 92121 http://rana.usc.edu:8376/~wisun/
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