ER / cytoplasmic fraction preparation

[Michael Daws]

Does anybody have a good protocol for preparing an endoplasmic reticulum
fraction from cell lysate, or alternatively for preparing a clean
cytoplasmic prep, free from cell membrane contamination?  Thanks for
your help.

Mike
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 Michael R. Daws, PhD 
 Umea Center for Molecular Pathogenesis
 901 87 Umea, Sweden
 Phone:   46-90-17-67-93       
 Fax:  46-90-77-80-07  
 E-mail:   Michael.Daws at ucmp.umu.se 
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