ER / cytoplasmic fraction preparation

Michael Daws michael.daws at
Wed Jan 21 11:59:00 EST 1998

Does anybody have a good protocol for preparing an endoplasmic reticulum
fraction from cell lysate, or alternatively for preparing a clean
cytoplasmic prep, free from cell membrane contamination?  Thanks for
your help.

 Michael R. Daws, PhD 
 Umea Center for Molecular Pathogenesis
 901 87 Umea, Sweden
 Phone:   46-90-17-67-93       
 Fax:  46-90-77-80-07  
 E-mail:   Michael.Daws at 

There are two types of people in this world, good and bad.  The good
sleep better, but the bad seem to enjoy the waking hours much
more.....W. Allen

More information about the Methods mailing list