96 well Coli cultures

Michael O'Donohue odonohue at lille.inra.fr
Wed Jan 21 06:29:28 EST 1998


Hi! Does anyone have any experience with E.coli culturing in 96 well
microtitre plates. Specifically, I would like to know how one can most
efficiently and reproducibly lyse E. coli in the wells. I imagine a
lysosyme step is the most useful but would an ultrasound water bath
help? Secondly do the cultures grow well in 96 well plates, good enough
for protein expression work that is?

Thanks for any information related to my question.



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