Electrotransfer of high MW proteins
Joel.Baguet at ens-lyon.fr
Wed Jan 21 10:42:02 EST 1998
I agree with this but I think there's an other buffer which works very
well it's the Bjerrum and Schafer-Nielsen buffer (48 mM Tris, 39 mM
glycine, pH 9.2, 0.0375 %SDS).
Moreover, in my experience, the western blots are very good when you
add 20% glycerol in the separating gel. In this way you can put the
stacking gel on the separating gel immediatly without waiting
separating gel acrylamide polymerization.
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