detecting RNA AND protein from same gel/filter

bongo moeller at CGL.UCSF.EDU
Thu Jan 22 19:24:05 EST 1998


Has anyone tried detecting/analyzing UV crosslinked RNA and protein on 
the same gel.  After electrophoresis I would like to transfer these 
complexes onto a filter and probe for both protein and RNA, if 
possible, to try and identify the protein (via antibody) crosslinked 
to a specific message(via hot probe). (Sort of a "NorthWestern")  
These samples are from in vivo transcription reactions. Any 
suggestions?  :)

Julie
please reply to juliej at cgl.ucsf.edu



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