tRNA isolation

zeleznik at zeleznik at
Thu Jan 22 13:59:32 EST 1998

I am looking for a protocol for a large scale purification 
of tRNA molecules from E.coli.  I have a protocol from 
Klemers et al. (1971) Methods in Enzymology, but I am 
having some trouble with it, so any other protocols or 
suggestions would be greatly appreciated.
thanks, matt zeleznik

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