In situ ...

Timur Yarovinsky tyarovin at blue.weeg.uiowa.edu
Thu Jan 22 14:54:18 EST 1998


Hi,

I need to localize particular mRNA in tissue by in situ hybridization. I 
see most authors use cRNA. Is probing with DNA labeled by nick translation
too bad (insensitive) for that? What about oligonucleotides? Do I really
need to make riboprobes if the mRNA is not overexpressed?

Is in situ RT-PCR more sensitive than hybridization with riboprobes?

Any comments welcome.


Timur Yarovinsky, Ph.D., M.D.
Oakdale Research Park, B141 MTF
2501 Crosspark Road
Coralville IA 52241-8802

tel.    (319) 335-4612
fax     (319) 335-4347
e-mail: timur-yarovinsky at uiowa.edu






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