In situ ...
tyarovin at blue.weeg.uiowa.edu
Thu Jan 22 14:54:18 EST 1998
I need to localize particular mRNA in tissue by in situ hybridization. I
see most authors use cRNA. Is probing with DNA labeled by nick translation
too bad (insensitive) for that? What about oligonucleotides? Do I really
need to make riboprobes if the mRNA is not overexpressed?
Is in situ RT-PCR more sensitive than hybridization with riboprobes?
Any comments welcome.
Timur Yarovinsky, Ph.D., M.D.
Oakdale Research Park, B141 MTF
2501 Crosspark Road
Coralville IA 52241-8802
tel. (319) 335-4612
fax (319) 335-4347
e-mail: timur-yarovinsky at uiowa.edu
More information about the Methods