Expression of toxic protein

Lyle Najita ijiwaru at WHEEL.DCN.DAVIS.CA.US
Fri Jan 23 18:17:36 EST 1998


On Fri, 23 Jan 1998, DOCTORHIM wrote:

> Glucose won't catabolite repress DE3 because  the lac promoter running the
> polymerase is not catabolite repressible.  
> 
I forgot that T7 RNA polymerase on DE3 lysogen is on lacUV5, thanks for
reminding me.  other alternative is to use Invitrogen's T7 pol M13 phage
and a F' host for expression.

Lyle




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