Electrotransfer of high MW proteins

Tom Vink tvink at xs4all.nl
Fri Jan 23 05:51:53 EST 1998


On Mon, 19 Jan 1998 17:55:42 +0000, Mrta Grifman
<mirtag at leonardo.ls.huji.ac.il> wrote:

>I am trying to obtain an efficient way to electrotransfer high MW
>proteins (100-200Kd) using a semi-dry blotter. I have tryied several
>procedures with no success. I would be grateful if someone could send me
>a good protocol 
>Thanks a lot!!
We most times use the phastgel system of Pharmacia and use so called
diffusion blotting,. This seems to work really fine for large
proteins. Especially a protein called von willebrand factor with
multimers upto 20 million can be transferred. 



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