Help: How to reduce autofluorescence in brain sections?

Ulrike Gausmann ugau at MTI-N.MTI.UNI-JENA.DE
Fri Jan 23 05:00:42 EST 1998


Since we try to perform immunofluorescence with brain
tissue (cryosections) fixed with formaldehyde 
autofluorescence is always a problem - doesn't matter
which wavelength we use. I tested the treatment with
sodium borohydride but there is no difference to the
untreated sections.
Does anybody know a method how to reduce the fluorescent
background?

Any hints are welcome,
Ulrike

-- 
Ulrike Gausmann			| D-07743 Jena
Institut fuer Anatomie		| Germany
Anatomie II			| Tel +49 (0)3641 938553
Teichgraben 7			| email ugau at mti-n.uni-jena.de



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