Your experience with RDA?

Mike Holloway Nospam at send_spam_to.Agis.com
Mon Jan 26 13:23:05 EST 1998


I'm attempting genomic RDA to discover homozygous deletion.  Several others
in our lab are not producing discreet amplified bands in agarose gels, but
instead cloning smears at the end of three cycles, and screening hundreds of
clones from this sublibrary.  Has anyone else used this technique in a
similar way?  My concern is that informative clones may represent a very
small subset of this library.

Thanks,
Mike Holloway
holloway at concentric.net



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