C-term his6 tag

Harold J. Drabkin hdrabkin at user1.channel1.com
Tue Jan 27 11:53:11 EST 1998

I was wondering if anyone has had the following experience expressing
C-terminal his6 tagged reporters in mammalian cells vs. E.coli

1. Drastic reduction in expression in mammalian cells compared to the
non-tagged version.  No problem in coli. (same reporter)
2. LOSS of the his6 tag in mammalian cells but not in E.coli  (same

thanks in advance

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