C-term his6 tag

Harold J. Drabkin hdrabkin at user1.channel1.com
Tue Jan 27 11:53:11 EST 1998


I was wondering if anyone has had the following experience expressing
C-terminal his6 tagged reporters in mammalian cells vs. E.coli

1. Drastic reduction in expression in mammalian cells compared to the
non-tagged version.  No problem in coli. (same reporter)
2. LOSS of the his6 tag in mammalian cells but not in E.coli  (same
reporter).

thanks in advance




More information about the Methods mailing list