protein precipitation

Peter Pediaditakis pxpst2 at vms.cis.pitt.edu
Wed Jan 28 13:40:34 EST 1998


In article <34CF5F8C.6545 at csulb.edu>, "Jeffrey A. Cohlberg"
<cohlberg at csulb.edu> wrote:

> RAY MCGUIRK (BIOCHEMISTRY) PG wrote:
> > 
> > I am looking for a protocol to precipitate total glycoprotein from
> > an aqueous solution of glycoprotein but I cannot use TCA as it will
> > cleave off the sugar moieties on the protein. A procedure using cold
> > ethanol or cold methanol which does not chemically cleave the protein
> > would be very useful.
> Try the method of Wessel and Flügge (1984) Analytical Biochemistry 138, 
> 141-143 -- a method which does not use TCA which we have found very 
> efficient

Phenol ether is even better and can work very well with extremely dilute
proteins.

-- 
"Don't you eat that yellow snow
    WAtch out where the Huskies go"

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