Question on Streptococcus pneumoniae

Ulrich Maier maier_hml at muenster.netsurf.de
Thu Jan 29 10:53:37 EST 1998


Richard L <lm11no at gpu.srv.ualberta.ca> (rm no) wrote:

>Hi there,
>
>I used to PCR E.coli colones with the method someone posted here
>before. But now I'm going to PCR a S.pneumoniae colony, I wonder if I can
>use the same method - just boiling the colony?
>
>Thanks a lot
>
>Richard
>

You can use the same method IMHO with nearly all bacterias.

Be carefull if you cultivate the mo´s on blood agar, the agar inhibits
the PCR.

Yours,

Uli Maier
(molecular biologist in a microbiologial-diagnostic lab.) 




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