stripping RNA probes

Judi 9320393 at maties.sun.ac.za
Thu Jan 29 16:54:55 EST 1998


I would like to start hybridizations using RNA probes labelled with DIG
or P-32 but have heard from the previous person who tried it in our lab
that they do not stipp off the membrane very easily. I have to have a
method where I can stripp membranes nicely because I have to probe my
membranes with quite a few probes. I really wouldn't like to run a
million Northerns!!
If anyone has any experience with this please help.
Thanks
Judi




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