His-tagged protein, urea & antibody production

Glenda Lawrence glendal at westmed.wh.usyd.edu.au
Thu Jan 29 22:18:18 EST 1998


Does anyone have ideas or experience re- the best
way to remove urea before injecting recombinant
protein into rabbits for antibody production?
How much urea can be tolerated?
The protein is a His-tagged protein (insoluble) purified
and eluted in 8M urea, 20mM Tris, 150mM NaCl and 200mM imidazole.


Glenda Lawrence

More information about the Methods mailing list