His-tagged protein, urea & antibody production

g. lawrence glendal at westmed.wh.usyd.edu.au
Thu Jan 29 22:14:58 EST 1998


Hi.
Does anyone have ideas or experience re- the best
way to remove urea before injecting recombinant
protein into rabbits for antibody production?
How much urea can be tolerated?
The protein is a his-tagged protein (insoluble) purified
and eluted in 8M urea, 20mM Tris, 150mM NaCl and 200mM imadazole.

Thanks,

Glenda Lawrence



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