intein system

[Roland Saldanha]

In article <6apd51$in8 at mserv1.dl.ac.uk>, "Hans-Helmut Niller"
<hans-helmut.niller at klinik.uni-regensburg.de> wrote:

> Hi netters,
> 
> who is having experiences with the Impact T7 system of NEB? Does it 
> work as a multi purpose expression and purification tool?


We have been quite happy with it - it works as advertised i.e. one step
near homogeneity.  I would highly recomend it.  In the version we used
they had a Tac promoter (not T7).  But we have swapped in T7 whenever we
wanted to.

Roland Saldanha.