intein system

Roland Saldanha rsaldanh at
Thu Jan 29 14:42:29 EST 1998

In article <6apd51$in8 at>, "Hans-Helmut Niller"
<hans-helmut.niller at> wrote:

> Hi netters,
> who is having experiences with the Impact T7 system of NEB? Does it 
> work as a multi purpose expression and purification tool?

We have been quite happy with it - it works as advertised i.e. one step
near homogeneity.  I would highly recomend it.  In the version we used
they had a Tac promoter (not T7).  But we have swapped in T7 whenever we
wanted to.

Roland Saldanha.

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