DD RT-PCR

fx.maquart fx.maquart at univ-reims.fr
Sat Jan 31 07:31:26 EST 1998


Hi Netters,

I try to run differential display, and I have the difficulties with PCR.
I obtain a number of bands on acrylamide gel but all of them
are between 500 - 1 000 bp. No product of low length.
What may be wrong?
Another question is about the purification degree of the oligos.
May it influence the PCR profile. No doscussion about it in
the literature. 
'Will be glad to read the oppinion of the experts.
Thank you.

Yanusz at :
yanusz.wegrowski at cleo.univ-reims.fr



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