Methods January 1998 Archives by author

Messages sorted by: [ thread ] [ subject ] [ date ] Index

Starting: Thu Jan 1 04:46:56 EST 1998
Ending: Sat Jan 31 23:36:35 EST 1998
Messages: 709

Help w/ Ni column protein purification Dana Nojima (CBN)
[Q]help-TA cloning after PCR 601
help 池野　聡一
invitro translation A.Habel
RPA--suggestions about which are the best kits. AO
magnetic beads markku Aalto
Agarose gel melts at room temp? Alex Abbas
BIOSCI/bionet miniFAQ & Fundraiser BIOSCI Administrator
touchdown PCR using pfu polymerase Jun-Ichi Aikawa
Custom service of scrrening of mouse genomic BAC library Jun-Ichi Aikawa
textbooks of BAC and PAC genomic library Jun-Ichi Aikawa
What is the best kit for mRNA purification? Jun-Ichi Aikawa
Better chelator of zinc than EDTA? Ashok Aiyar
storage of westerns Luis Alvarez
tissue culture solutions Shlomo Amir
Endotoxin removal Mike Amstutz
Expression of toxic protein Chen Ho An
Commerical Bacculovirus Service?? Scott Andrews
NdeI Scott Andrews
Commerical Bacculovirus Service?? Scott Andrews
New Section in AgBiotech Reporter Andrew R. Apel
Excerpt from AgBiotech Reporter - Call for Submissions Andrew R. Apel
DIG In Situs Asim
Tm of RNA/RNA hybrids Mark Asnicar
Commercial source for SV40 large T-ag? René Assenberg
protein-DNA coimmunoprecipitation Flore BELLEFONTAINE
agarose gel electrophoresis conditions Joseph C. Bagshaw
Lambda zap V lambda dash Joseph C. Bagshaw
site-specific phosphorylation Joel Baguet
in vivo chemical cross-linkers Joel Baguet
RNA extraction from liver Joel Baguet
Electrotransfer of high MW proteins Joel Baguet
STABILITY of solubilization buffer Rocky Baker
Dye terminator composition???? Wayne M. Barnes
? E.Coli competent greater than 10 +E 8 without electroporation Tim Barnett
gfp-neo fusion Matthew D. Beasley
E. coli. strain B834 commercially available? Jakob Begun
Plasmid Isolation from Yeast Matt Benson
Invitro trascription vector with poly AA? Hallgeir Bergum
plant sulfur metabolism Oliver Berkowitz
SPA (Single Primer Amplification) Yves Bertheau
best way to isolate bands from gels Bike&Linux
precipitation of very small DNA fragments Ransom Hill Bioscience
HPLC for Protein Separation and Identification Advanced Biotechnologies
mtDNA invertebrates universal primers Black, Michael
New SDS-PAGE / Western Blotting Review Shaun D. Black
Electrotransfer of high MW proteins Shaun D. Black
in vitro transcription Darren Boehning
GST fusion protein solubility Frederik Boernke
best 2 hybrid system Frederik Boernke
BLASTn vs. BLASTp Frederik Boernke
Reprobing of western blot Frederik Boernke
2hybrid:Expression level of bait vs prey Frederik Boernke
His-tagged protein, urea & antibody production Frederik Boernke
oligo dT column too slow Kent S. Boles
Gibco RNAse mix?? Peta C. Bonham-Smith
Agarose gel melts at room temp Cres Bookstein
ddisplay Cres Bookstein
Qiagen's California address J. Boyce
Thermostable RT J. Boyce
JM108 Joan Boyce
southern enigma Chris Boyd
Mac molbio software recommendations? Charles Brenner
DD-RT-PCR Brian
silver stain; sensitivity RNA vs. protein Juergen Brosius
New World Monkey genomic library Juergen Brosius
Test Bubs
Who makes MACS columns? Sebastian Bunka
[Q]: RT-PCR 1st strand kit Sebastian Bunka
vector replicon John E. Butler
sequencing Neil Butt
SDSPAGE Problems Dr E. Buxbaum
SSCP Gregory Buzard
Address for making nucleotides C.L.L.
NdeI C.L.L.
Q:QuickChange and deletions CCoburn
precipitation of very small DNA fragments CCoburn
Tm and Hybridization temperature CVRL
(none) Mr. Daniel Cadolle
(none) Mr. Daniel Cadolle
polyclar-AT Bruce A. Caldwell
Update on Used Lab Equipment Site James J. Campanella
c-myc in plants Jan Carette
best way to isolate bands from gels Ed Castro
first time the first... Cattaruzza at veg-physiol.med.uni-goettingen.de
Chr 12 markers help Robert B. Chadwick
ABI Linkage Mapping Set 2? Robert B. Chadwick
E. coli. strain B834 commercially available? Zhonglin Chai
Is a 15 na oligo ok for colony hybridization? Tom Chappell
help:clone a large fragment ChemicalSuperFreak
In situ hybridization in pathology ChemicalSuperFreak
best way to isolate bands from gels ChemicalSuperFreak
help please...I need to buy equipment..and I dont know what to choose.. ChemicalSuperFreak
DD-RT-PCR Lawrence Chen
µM -- please check my calculation Lawrence Chen
[Q]help-TA cloning after PCR Dr. Duncan Clark
Amplification of cDNA inserts from freeze-thawed phage Dr. Duncan Clark
help: genomic DNA extraction from tissue Dr. Duncan Clark
protein precipitation Jeffrey A. Cohlberg
Km values handbook? Jeffrey A. Cohlberg
plastic-binding phage Borg Collective
Q: Choccolate-like smelling bugs? Martin Collinson
pH of Tris Martin Collinson
Pharmaceutical XRD Data plus warning for International Quant Analysis Round Robin participants Lachlan Cranswick
Alternative Polyadnylation sites Carmel Curtis
HELP!!!white bands on my autoradiogram! D.L.Clarke
Detection of early transcripts? References required? D.L.Clarke
DD-RT-PCR D.L.Clarke
[Q]help-TA cloning after PCR DOCTORHIM
Expression of toxic protein DOCTORHIM
Cloned beta-galactosidase from Strep. pneumonia DOCTORHIM
What size tip for Sonifier? DOCTORHIM at AOL.COM
Better chelator of zinc than EDTA? DOCTORHIM at AOL.COM
CMF hanks buffer Mathias DREXLER
image analysers Jo Daniels
NP40 (was Re: nucleus removal in Sf9 cells.) David L. Haviland, Ph.D.
oligo dT column too slow David L. Haviland, Ph.D.
looking for gamma glutamyl transferase antibody Max Davidson
E. coli Cu++ Sensitivity? Rick Davis
ER/cytoplasmic prep Michael Daws
ER / cytoplasmic fraction preparation Michael Daws
Tet-ON vector(s) Patrizia Dell'Era
Ammonium Acetate 'going bad?' Alex Dobrovic
help: genomic DNA extraction from tissue Alex Dobrovic
Redissolvinging lyophilised DNA problems Alex Dobrovic
SDSPAGE Problems Fergus Doherty
DNA-Protein Cross Linking Methods Sudhanshu Dole
BM's DNA binding protein purification kit. Sudhanshu Dole
Boehringer M.'s DNA binding protein purification kit. Sudhanshu Dole
Ammonium Acetate 'going bad?' Mark Dowton
C-term his6 tag Harold J. Drabkin
tRNA isolation Harold J. Drabkin
Oligo hybridization. Harold J. Drabkin
Will Dpn I cut half-methylated DNA? Richard J. Dudley
Any experience with TOPO PCR cloning kit? Richard J. Dudley
site directed mutagenesis Richard J. Dudley
JM110 Richard J. Dudley
Invitro trascription vector with poly AA? Richard J. Dudley
fabrication of nylon membrane discontinued Edith Duerbaum
Commercial source of full length cDNA clones. Donald Dunbar
Correct sizing of alleles after silver staining of polyacrylamide gels E.C.WATSON at lboro.ac.uk
Salmonella gDNA library Ann Eakin
pET-vector Christian Ebeling
T7 RNA polymerase phage? Christian Ebeling
CSL Company - HELP!!! Dianne Emslie
Refridgerated Microcentrifuges. Marie Estock
T-DNA plasmid required urgently Fornavn Etternavn
Fission Yeast protocols on WWWeb Hochstenbach F
Isolation of cell membranes from tisue culture cells. Judith Blasser F-H
storage of westerns Frank O. Fackelmayer
Amplification of cDNA inserts from freeze-thawed phage Frank O. Fackelmayer
precipitation of very small DNA fragments Frank O. Fackelmayer
E. coli Cu++ Sensitivity? Frank O. Fackelmayer
EtBr vs 32P in RT-PCR Ross Feldberg
Poliovirus temperature stability? Karl Fischer
Hybridization enhancement Karl Fischer
southern enigma Karl Fischer
Follow-up : hybridization enhancement Karl Fischer
Addendum: Hybridization enhancement Karl Fischer
Histochemical techniques Tim Fitzmaurice
probe labeling Michael Flores
Gibco RNAse mix?? Bryan L. Ford
DNA isolation from hair ChemicalSuper Freak
[Q]help-TA cloning after PCR Roderic Fuerst
site directed mutagenesis ANNE GEORGE
PVPP Vijay Gadkar
Western Blot Problem Daniela Gadotti
ddisplay Mr. J Gaughan
Help: How to reduce autofluorescence in brain sections? Ulrike Gausmann
Any experience with TOPO PCR cloning kit? Yoram Gerchman
sex-specific labelling of cells? Nicholas J. Gibson
Bird sexing using feather as the source of DNA Gizbab
Looking for Rho small G proteins Ole Gjoerup
Disposable Oak Ridge tubes Tom Glenn
Better chelator of zinc than EDTA? Damaged Goods
differential display equipment Michael C. Gorry
Best cell line for expression cloning but not COS-7 c David de Graaf
Best cell line for expression cloning but not COS-7 c David de Graaf
genotyping SCID and Nu/Nu mice David de Graaf
genotyping SCID and Nu/Nu mice David de Graaf
Q: Choccolate-like smelling bugs? Roney Graf
Yeast Plasmid Isolation Richard Grant
Double insert ligation Richard Grant
NdeI Richard P. Grant
Electrotransfer of high MW proteins Mrta Grifman
DIG In Situs Paul C. Grimm
Best cell line for expression cloning but not COS-7 ? Christophe Grundschober
Yeast strains question Zé Guerra
Protocol for Hydroxy Radical DNA-Footprints Marco Gymnopoulos
Cloned beta-galactosidase from Strep. pneumonia HMiller774
RPA or RT-PCR HRIND
Q:QuickChange and deletions Hadi
Magnetic Carriers Meeting - CALL FOR ABSTRACTS Urs Hafeli
silence a gene by insertion of an antibiotic cassette R.D. Haigh
re-use of electroporation cuvettes R.D. Haigh
best 2 hybrid system Dimitri Harmegnies
mtDNA invertebrates universal primers HaroldB786
DNA isolation from hair HaroldB786
need bacteria TOP10/P3 or pcDNAI-Amp Said Hashemolhosseini
RNA isolation from tissue perfused w/UW solution Randy Haun
Gene Hunter company's add. Randy Haun
Invitro trascription vector with poly AA? Randy Haun
T-DNA plasmid required urgently J. Hendriks
RNA extraction from liver J. Hendriks
elisa reader filter needed ANY HINTS ? / SLT lab instr. Guy Hermans
EMSA optimization? Mirkka Herranen
CD13 antibodies Michael J. Herron
in vitro transcription Warren Hirst
RNA extraction from liver Warren Hirst
Help w/ Ni column protein purification Michael Hodges
prediction of protein-protein contact sites Carsten Hohoff
Q:genomic DNA extraction AKi Hoji
Protinase K long-term storage, again AKi Hoji
Help:Mycoplasm contamination in Vaccinia soup AKi Hoji
SDS-PAGE gel-shift to look at protein phosphorylation Brian Hollander
Genomic RDA? Mike Holloway
Your experience with RDA? Mike Holloway
pH of Tris Lou Hom
Problems with restriction digests Hopkins, Charlotte
Postdoc Position Hoyun_Lee at cancercare.on.ca
Ultra-low temp. freezer Hoyun_Lee at cancercare.on.ca
No plasmid form transformed BL21(DE3) Ke-Xue Huang
software for searching repeat sequence Ke-Xue Huang
No plasmid from trasformed BL21(DE3) Ke-Xue Huang
Mac molbio software recommendations? Jeff Huckaby
1D-PAGE software: free/shareware?? Jeff Huckaby
Software for Sequencing Projects? Jeff Huckaby
Many questions Jürgen Häußler
Lambda Zap v/s Dash Ramaswamy K. Iyer
Western Blot Problem Ramaswamy K. Iyer
ECACC Fraser Dr N J
ECACC Fraser Dr N J
Gene Hunter company's add. VASANTHARAJAN JANAKIRAMAN
help:clone a large fragment Christophe Jacob
Endotoxin removal James
Stable cell transfection James
Protein purification using guanidinum hydrochloride James
DNA software for Linux Keith James
Low-protease strain of B. subtilis Arul Jayaraman
What's the difference between homogenisation and sonication? Chew Sek Jin
B-galactosidase inhibitor Dan Johnson
Lambda zap V lambda dash Richard Johnson
GST fusion protein solubility Keith Jolley
Help w/ Ni column protein purification Keith Jolley
Help: PAGE gels cracking Zophonias O. Jonsson
stripping RNA probes Judi
GAL-lacZ plasmid for yeast? Justinian
protein purification K.G.Spink
Stable cell transfection Hendrie Kain
elisa reader filter needed ANY HINTS ? / SLT lab instr. Bob Kays
QuickHyb and Nonisotopic hybridization Bernd Kazmierczak
Help w/ Ni column protein purification Keith
Help optimize electroporation Jayati Kesavan
protein recovery Anwar A. Khan
Expressing the cpp32 in yeast nucleus Dooyeon Kim
Expressing the cpp32 in yeast nucleus Dooyeon Kim
Help!!>> DNA Replacement Hynumin Kim
Que] about Conjugation? Hyunmin Kim
genotyping SCID and Nu/Nu mice Jorg Kirberg
genotyping SCID and Nu/Nu mice Jorg Kirberg
qe: re-use of electroporation cuvettes Rob Kirkpatrick
ELISA plate readers Susanne Kjemtrup
Ph.D. student position/Sweden (plant mol. biol.) Leszek Andrzej Kleczkowski
DNA invitro-assay Nikolai Klein
Help optimize electroporation Dima Klenchin
Refridgerated Microcentrifuges. => buy rotor (Beckman JA-18.1) for your existing centrifuge instead Dima Klenchin
Help w/ Ni column protein purification Dima Klenchin
Help w/ Ni column protein purification Dima Klenchin
Calibration of fura-2 Dr. K. Kluge
Predicting Coiled Coils from Protein Sequences Alex Knight
Gene Hunter company's add. Glenn Knight
Help w/ Ni column protein purification Lars Komorowski
Ni-NTA again Lars Komorowski
E. coli Cu++ Sensitivity? Lars Komorowski
coating microscope coverslips with streptavidin Jonas Korlach
DNA isolation from hair John Korte
No plasmid from trasformed BL21(DE3) Cornelius Krasel
total protein extraction for FPLC Cornelius Krasel
Km values handbook? Georg Kroeger
software for finding restriction sites Rork D. Kuick
Problems with restriction digests Richard L
Question on Streptococcus pneumoniae Richard L
probe labeling Lab
pMOB plasmid sequence Wood Lab
genomic PCR help John Ladasky
LA-PCR problems John Ladasky
Transfection problems John Ladasky
Inverse PCR -- degraded DNA ends? John Ladasky
DNA isolation from hair John Ladasky
quantif. of DNA/RNA copy nbrs for Q-PCR. Ladriere
Chlamydia Pneumoniae Ladriere
Differential display problems John Landers
TRINUCLEOTIDE REPEATS James W. Larkin
Any experience with TOPO PCR cloning kit? Michael Lassner
His-tagged protein, urea & antibody production Glenda Lawrence
elisa reader filter needed ANY HINTS ? / SLT lab instr. Erich Lederer
non-radioactive RPA Clair Lee
Commerical Bacculovirus Service?? Sam Lee
low copy origin for expression plasmid Tosso Leeb
OK to send e-mail? Andrew Leech
elisa reader filter needed ANY HINTS ? / SLT lab instr. T. Lenhard
Double insert ligation Victor Levenson
Poliovirus temperature stability? David N. Levy
Who makes MACS columns? David N. Levy
Help: Density of a typical bacterial cell ( eg. E. Coli ) Bin Lin
Pre-cast SDS-PAGE gels for Hoeffer Jeffrey Lin
[2hybrid] HF7c [URA-] ? Pierre Lindenbaum
GEX-GST induction Pierre Lindenbaum
What do you think of this CIP Pierre Lindenbaum
pYX212 plasmid sequence ? Pierre Lindenbaum
protein-protein interaction Lion
Double insert ligation David C. Logan
HELP!!!white bands on my autoradiogram! LouPass
ELISA and Alkaline phosphatase-DNA probes: How to calculate signal:noise? Cytocell Ltd.
Alkaline phosphatase-DNA probes: How to calculate signal:noise? Cytocell Ltd.
Best method for freezing tissue for RT-PCR Margaret Koziel MD
GT repeat characterization GR Taylor PhD MRCPath
BESS T-Scan Kit for Mutation Detection???? GR Taylor PhD MRCPath
C-term labeling of a peptide ? Josef P. Magyar
DNA from serum Ulrich Maier
Question on Streptococcus pneumoniae Ulrich Maier
Plasmid/help Manash
dNTP problems -Reply Kathy Mangold
Double insert ligation Ned Mantei
Best cell line for expression cloning but not COS-7 ? Ned Mantei
IEF Stephen Marek
storage of bacterial plates Tulene McCabe
protein precipitation Ian McFarlane
Volatile buffer pH7.5? Ian McFarlane
non-radioactive detection kit Bill McKendree
plant expression vector Bill McKendree
Any experience with TOPO PCR cloning kit? Bill McKendree
Linear vs. supercoiled DNA in plasmid transfection Jose E. Mejia
Help: Density of a typical bacterial cell ( eg. E. Coli ) Pascal Mertens
Background plaques when screening Lambda ZAP II genomic libraries with DNA probe Michael
Q: Choccolate-like smelling bugs? Phillip San Miguel
DNA structure and PFGE Phillip San Miguel
Gangliosides - Bachem? Erik A.J. Miljan
1D-PAGE software: free/shareware?? Jay Miller
Hifax Wong Yau Ming
in vivo chemical cross-linkers Souren Mkrtchian
No plasmid form transformed BL21(DE3) Kevin A. Morano
Help! Antibodies! Mr. G. Morley
Q:Transfecting breast cancer cell lines? jo Morris
Small protein Western Analysis Per Mygind
JM110 Per Mygind
Chlamydia Pneumoniae Per Mygind
Expression of toxic protein Lyle Najita
Who makes MACS columns? Darren A. Natale
Help w/ Ni column protein purification Darren A. Natale
Need PFGE apparatus suppliers David Neece
total protein extraction for FPLC John A. Newitt
intein system Hans-Helmut Niller
Oligo hybridization. Peter Nilsson
Ultra-low temp. freezer Pamela Norton
96 well Coli cultures Michael O'Donohue
NdeI Michael O'Donohue
Protein-protein ERNESTO OVIDEO ORTA
Epitope Mapping Software [Q] Michael Oda
storage of westerns Martin Offterdinger
Easiest Method of Synchronizing CHO Cells Martin Offterdinger
SDS-PAGE gel-shift to look at protein phosphorylation Martin Offterdinger
Q:Transfecting breast cancer cell lines? Martin Offterdinger
in vitro transcription Martin Offterdinger
library screening for secreted proteins Dr GC Oliveira
A minimal medium for S. aureus RN4220 Ralf Oltmanns
Chlamydia Pneumoniae J.M. Ossewaarde
lectin for O-linked sugars RAY MCGUIRK (BIOCHEMISTRY) PG
protein precipitation RAY MCGUIRK (BIOCHEMISTRY) PG
Volatile buffer pH7.5? Laurent POULOUIN
genomic PCR help Jennifer Pan
LA-PCR problems Parent, Eric
LA PCR Parent, Eric
mtDNA invertebrates universal primers Parent, Eric
YEP357 Lestrate Pascal
DNA labeling with fluorescein Lestrate Pascal
site directed mutagenesis Lestrate Pascal
SDS-PAGE gel-shift to look at protein phosphorylation John Pawlowski
Electrotransfer of high MW proteins PbZep
Spreadsheet invention Dan Pedersen
protein precipitation Peter Pediaditakis
(none) Dan Pelah
non-radioactive detection kit Dan Pelah
CV-1 genomic library Richard Pelletier
storage of westerns Marisol Rodriguez Pena
Which is better: RPA or RT-PCR Marisol Rodriguez Pena
<no subject> Marisol Rodriguez Pena
Immunoprecipitation Marisol Rodriguez Pena
[Q]help-TA cloning after PCR Jiqing Peng
Dye for Sephadex column Pete's Person
AFLPs using S35? Pete's Person
help Christian Petzelt
Hyaluronic acid:Extraction, Purification, Sterilization Thanit Pewnim
HELP! Wizard genomic purification kit Jennifer Philhower
Melting temp formula for RNA:RNA Andy Phillips
Melting temp formula for RNA:RNA Andy Phillips
Tm of RNA/RNA hybrids Andy Phillips
Melting temp formula for RNA:RNA (sorry) Andy Phillips
help:clone a large fragment Andy Phillips
BLASTn vs. BLASTp Andy Phillips
Tm and Hybridization temperature Andy Phillips
T7 RNA polymerase phage? Brad Pietz
[Q] Anti-p53(Bovine) antibody info's Martina Plass
Vertical electrophoresis unit Anton Posch
2-D PAGE training - attend a 3 days course in Cologne, Germany Anton Posch
Stable cell transfection Kiley R. Prilliman
PCR gradient thermocyclers Kiley R. Prilliman
purification of CD3, CD14 and CD19 cells Sylvie Provost
best way to extract DNA from gel QGUO1
Yeast Plasmid prep QGUO1
Is a 15 na oligo ok for colony hybridization? REWHLAB
Small protein Western Analysis Christian Radauer
Problems with restriction digests Keith Rand
Source for digitonin? Nicole Rapior
lectin for O-linked sugars Nicole Rapior
Losing the plasmid Mary P. Remington
What size tip for Sonifier? Mary P. Remington
Metabolic labeling of human monocytes Blair R. Renshaw
Sperm and RNA isolation Rentia
Transfection problems Rentia
T7 RNA polymerase phage? Wolfgang Resch
Vectors for testing promoters in plants Steve Rice
cleavable tags Cheryl L. Richards
storage of westerns John Richburg
FasL mono, di-, and trimers John Richburg
Custom Transfection Michael Rist
Adenoviral Titer Protocols R. Brooks Robey
cloning woes Robot-Boy
CMF hanks buffer Paul R. Rohde
DD-RT-PCR Paul R. Rohde
site directed mutagenesis Paul R. Rohde
site directed mutagenesis Paul R. Rohde
RNA isolation from tissue perfused w/UW solution Paul R. Rohde
Post-DD problem Maaria Roschier
best way to isolate bands from gels Michael Rosemann
ECACC Michael Rosemann
Problems with restriction digests Michael Rosemann
HELP! Wizard genomic purification kit Hiranya Roychowdhury
PVPP Hiranya Roychowdhury
STABILITY of solubilization buffer Hiranya Roychowdhury
End conversion of PCR products Hiranya Roychowdhury
help JIANG SHOU/MICROBIOLOGY/IMMUNOLOGY
Please help JIANG SHOU/MICROBIOLOGY/IMMUNOLOGY
intein system Roland Saldanha
agarose gel electrophoresis conditions Tim Salmon
SDS-PAGE gel shift to look at protein phosphorylation Tim Salmon
Need PFGE apparatus suppliers Tony Sanchez
in vitro transcription Heinz J. Schaefers
total protein extraction for FPLC Heinz J. Schaefers
Will Dpn I cut half-methylated DNA? Wolfgang Schechinger
Endotoxin Removal Wolfgang Schechinger
FIAU -- Looking for source of FIAU (Ganciclovir) Wolfgang Schechinger
Q: EST Databases other than GDB? Wolfgang Schechinger
best way to isolate bands from gels Wolfgang Schechinger
best way to isolate bands from gels Wolfgang Schechinger
site directed mutagenesis Wolfgang Schechinger
Best cell line for expression cloning but not COS-7 c Wolfgang Schechinger
Q: Choccolate-like smelling bugs? Wolfgang Schechinger
Looking for Rho small G proteins Wolfgang Schechinger
software for finding restriction sites Wolfgang Schechinger
Electrotransfer of high MW proteins Wolfgang Schechinger
help:clone a large fragment Daniel Schlieper
ECL Sandra Schneider
(none) Sven Schoeppner
ECACC Axel Schumacher
In situ hybridization in pathology Andrew Scott
software for finding restriction sites Dr. Dirk Seegert
EMSA optimization? P.J. Shaw
antibody to trpE gene product? Regina Shaw
New Product for Gene Regulation Studies - FREE! Simon Sims
Double insert ligation Koen De Smet
GST fusion protein solubility Koen De Smet
GST fusion protein solubility Koen De Smet
PCR gradient thermocyclers Koen De Smet
Help w/ Ni column protein purification Koen De Smet
Small protein Western Analysis A P Smith
A minimal medium for S. aureus RN4220 Brian Smith
qe: re-use of electroporation cuvettes Harold E Smith
normalized cDNA libraries Harold E Smith
SEAP assay Mike Smith
Post-DD problem Mart Speek
southern enigma Mart Speek
southern enigma Mart Speek
Calcium Binding Proteins Symposium in Germany Ralf Spenneberg
Best cell line for expression cloning but not COS-7 ? Dom Spinella
Best cell line for expression cloning but not COS-7 c Dom Spinella
Best cell line for expression cloning but not COS-7 c Dom Spinella
[Q]help-TA cloning after PCR Dom Spinella
Best cell line for expression cloning but not COS-7 c Dom Spinella
Q: EST Databases other than GDB? Dom Spinella
plastic-binding phage Dom Spinella
Dye for Sephadex column Dom Spinella
C-term labeling of a peptide ? Dom Spinella
differential display equipmentPS Dom Spinella
Transfection problems Dom Spinella
6His protein Henri M.H. Spronk
SDS-PAGE gel-shift to look at protein phosphorylation Bob Steinberg
Serum Albumin on Platinum Joachim Steiner
Electrotransfer of high MW proteins Stephan
Software for Sequencing Projects? Stephan
References: Electrotransfer of high MW proteins Stephan
PCR gradient thermocyclers Frank Struyf
southern enigma William Sun
Southern Enigma William Sun
prediction of protein-protein contact sites Vladimir Svetlov
Q:QuickChange and deletions Vladimir Svetlov
Yeast Plasmid Isolation Vladimir Svetlov
Plasmid Isolation from Yeast Vladimir Svetlov
site directed mutagenesis Vladimir Svetlov
best way to isolate bands from gels Vladimir Svetlov
Help w/ Ni column protein purification Vladimir Svetlov
Q:QuickChange and deletions Vladimir Svetlov
NdeI Vladimir Svetlov
NdeI Vladimir Svetlov
6His protein Vladimir Svetlov
RPA or RT-PCR Caroline Szymeczek-Seay
dNTP problems TBott53
DNA Isolation Guy Tel-Zur
storage of westerns Brian S. Thoma
Expression of toxic protein Thomas
DNA proofreading and growth Matt Thomas
No plasmid from trasformed BL21(DE3) John R. Thompson
help please...I need to buy equipment..and I dont know what to choose.. Lars Thomsen
Patch-clamping transfected mammalian cell lines? Lars Thomsen
Is a 15 na oligo ok for colony hybridization? Timaoxin
PCR ribotyping with 16S-23S IGS Kirsty Townsend
pH-meter Michael J. Tuvim
in vitro transcription Mac Twocx
Commercial source of full length cDNA clones. Darren Tyson
Reprobing of western blot Darren Tyson
Electrotransfer of high MW proteins Takashi Umeyama
DNA structure and PFGE Edgar Valencia
DNA structure and PFGE Edgar Valencia
Amplification of cDNA inserts from freeze-thawed phage Jorge Vasquez-Kool
silence a gene by insertion of an antibiotic casette Marina Verkhovskaya
[Q]help-TA cloning after PCR Marina Verkhovskaya
RNA standard for quantitative rt-pcr Vidar
DNA from serum Jean-Pierre de Villartay
Electrotransfer of high MW proteins Tom Vink
Epitope Mapping Software [Q] Tom Vink
A Non high fidility 5.3K PCR? Svetlov Vladimir
GST fusion protein solubility Arnoud van Vliet
DNA labeling with fluorescein Arnoud van Vliet
Losing the plasmid Arnoud van Vliet
DNA Isolation Arnoud van Vliet
Better chelator of zinc than EDTA? Arnoud van Vliet
PCR gradient thermocyclers WSchick
What size tip for Sonifier? WSchick at AOL.COM
Partners wanted for dev't of new combinatorial ch'try method Sipke Wadman
proline not by HPLC? Kerstin Waigand
Histochemical techniques Michael William Walsh
FIAU -- Looking for source of FIAU Don Wang
library screening for secreted proteins Peter Wang
T7 RNA polymerase phage? Peter Wang
SSCP Beth Wapelhorst
probe labeling Beth Wapelhorst
stripping RNA probes Andrew Warkman
CSL Company - HELP!!! Krzysztof Wasowicz
Histochemical techniques Krzysztof Wasowicz
Volatile buffer pH7.5? Martin Webb
[2hybrid] HF7c [URA-] ? Ralf Weigel
beta actin Dr. Cora Weigert
S1 RNA Analysis January Weiner
DNA software for Linux January Weiner
Hybridization enhancement Mr. T. Weissensteiner
>DD-PCR problem Mr. T. Weissensteiner
ctab hyb enhancement Mr. T. Weissensteiner
How to separate bulged from perfect DNA duplexes? Malcolm White
Separation of bulged from perfect duplex DNAs Malcolm White
software for finding restriction sites C.S. Wilding
SeaLite Science AquaLite Wilkinson, Deborah
GST fusion protein solubility Randall C Willis
STABILITY of solubilization buffer Randall C Willis
Protein purification using guanidinum hydrochloride Randall C Willis
Low or No Background Cloning Wilson
qe: re-use of electroporation cuvettes Reinhard Windoffer
Southern Enigma David Winterbourne
Which is better: RPA or RT-PCR Harry Witchel
Endotoxin Removal Harry Witchel
Patch-clamping transfected mammalian cell lines? Harry Witchel
NP40 (was Re: nucleus removal in Sf9 cells.) Arthur Wohlwill
rat growth factor desperately wanted Peter Wollenberg
IEF Robert Woodward
Great News: Bioinformation Search Center Version 3.0 ! Mousheng Xu
invitro translation Xiao-Hua Xue
Expression of toxic protein Wan Seok Yang
detection of green fluorescent protein Yanghm
In situ ... Timur Yarovinsky
PCR cloning in phage Timur Yarovinsky
unsubscribe Seok-min Yoon
help Seok-min Yoon
NP40 (was Re: nucleus removal in Sf9 cells.) Ian A. York
Source for digitonin? Ian A. York
Best cell line for expression cloning but not COS-7 c Ian A. York
Best cell line for expression cloning but not COS-7 c Ian A. York
Best cell line for expression cloning but not COS-7 c Ian A. York
Stable cell transfection Ian A. York
NdeI Ian A. York
Double insert ligation T.J. Young
vacuole transport in pombe T.J. Young
18s rRNA probe Z.Huang
help: genomic DNA extraction from tissue Z.Huang
BLASTn vs. BLASTp Colby Zaph
bandshift in agarose gel Markus Zettl
18s rRNA probe Christopher Zhao
Ammonium Acetate 'going bad?' Marieke R. Koedood Zhao
storage of bacterial plates Marieke R. Koedood Zhao
Genomic library Z. Zhao
MBP fusion protein does not bind to amylose? ZhongLingChai
HPLC for Protein Separation and Identification yuanxiang Zhou
Electrophoresis, TLC, Image analysi aab at IX.NETCOM.COM
Lawsuit admin at alt.misc.news.legal
GT repeat characterization agabiti at master.cci.unibs.it
Genomic library fused to HIS3? aplummer at dax-atm.rz.uni-frankfurt.de
probe labeling as-sm at cyberus.ca
GST fusion protein solubility atanasio
Taq Extender - What is it? bbeitzel at biomail.ucsd.edu
unsubscribe beatrice.seguin at utoronto.ca
how do I quit methods beatrice.seguin at utoronto.ca
Bio-Rad's gene gun tubing coating bezstarosti at bc1.fgg.eur.nl
detecting RNA AND protein from same gel/filter bongo
test- please ignore john brennand
in vitro transcription brett
blue crab cDNA library marius brouwer
Plasmid Isolation from Yeast byudkin at bioch.ox.ak.uk
apo-protein of cyt c, isolation protocol... ckmsl at uohyd.ernet.in
conditions for DNA binding cll
need BL21A(DE3) strain of E. coli bipin k. dalmia
software for finding restriction sites deepakk at mailcity.com
Q:Transfecting breast cancer cell lines? delios97 at hotmail.com
3'end mapping patcharaporn deshnium
1D-PAGE software: free/shareware?? ecastro at eucmax.sim.ucm.es
software for finding restriction sites eizema at bc1.fgg.eur.nl
Reprobing of western blot eizema at bc1.fgg.eur.nl
ctab hyb enhancements elader at ambion.com
RNA from christmas trees elader at ambion.com
oligo dT column too slow elader at ambion.com
Home Bussiness esikes at ANET-DFW.COM
Software for Sequencing Projects? Crepineau florent
### $35 FOR $300 IN FREE FAST FOOD AND $$$ ### freefastfood at HOTMAIL.COM
Dye terminator composition???? fwhyde at ibm.net
New Stratagene Select cII Kit-details? fwhyde at ibm.net
BESS T-Scan Kit for Mutation Detection???? fwhyde at ibm.net
DD RT-PCR fx.maquart
End conversion of PCR products craig garen
OPTIMIZING RECOMBINATION TO OBTAIN STABLES paul gold
Easiest Method of Synchronizing CHO Cells paul gold
Theoretical question: Is recombination efficiency cell cycle dependent? paul gold
Ni-NTA preparation m griep
Q:QuickChange and deletions and native Pfu and cloned .... hadi
mouse beta-actin wiebke hallensleben
need RNA marker and Nytran. hongxiang hui
Endotoxin removal Administrador de iihema.fmed.uba.ar
Expression of toxic protein ijiwaru at dcn.davis.ca.us
Info immun
(none) immun
T7 RNA polymerase phage? is.bbsrc.ac.uk
Ustilago protocols jan.morren at bio.kuleuven.ac.be
HELLO jeskritt
problems in cell culture zhang jiping
CAT antibody kev
HELP!!!white bands on my autoradiogram! kong
RNA isolation from Xmas tree kp at everx.szbk.u-szeged.hu
software for finding restriction sites paul lacy
His-tagged protein, urea & antibody production g. lawrence
Help: PAGE gels cracking lee.285 at osu.edu
Low or No Background Cloning qin ling
genotyping SCID and Nu/Nu mice lr50916 at glaxowellcome.com
software for finding restriction sites lr50916 at glaxowellcome.com
3'end mapping lr50916 at glaxowellcome.com
Transfection problems lr50916 at glaxowellcome.com
Yeast Plasmid Isolation mabenson at technologist.com
normalized cDNA libraries mdraper at opal.tufts.edu
Phaseolus vulgaris genomic or leaf cDNA library mjohnson at biology.as.ua.edu
CSL Company - HELP!!! thu nhat thi nguyen
OK to send e-mail? owl at OWLSEYE.COM
Is Your Web Site A Secret? owl at OWLSEYE.COM
Is Your Web Site A Secret? owl at owlseye.com
Own Your Own Power Company!! powermlm1 at HOTMAIL.COM
? E.Coli competent greater than 10 +E 8 without electroporation reber at uni-bonn.de
Best cell line for expression cloning but not COS-7 c ronlab
Best cell line for expression cloning but not COS-7 c ronlab
Q: cultivation of ES and feeder cells. ronlab
in vitro transcription ryyukhan at FACSTAFF.WISC.EDU
Chr 12 markers Help s.pritchard
help: genomic DNA extraction from tissue s.pritchard
Q:genomic DNA extraction s.pritchard
for testing s952003 at MAILSERV.CUHK.EDU.HK
HELP: Protein overexpression using pTRXFus sathish
Help a student with science fair sean
Mouse ras and bcl2 primers snyderrf
Reprobing of western blot snyderrf
Gen bank tlibert
ECACC un691cs at genius.embnet.dkfz-heidelberg.de
Genuine **HOME-BASED** Employment Offer: up_yors11 at NETIMATION.COM
[Q]help-TA cloning after PCR user
Glycerol and Tm vilimf01 at mcrcr6.med.nyu.edu
request for join yanli wang
His-tag inhibits anti-myc-antibody wulf-dirk.leuschner at tuebingen.mpg.de
thin bands in non-reducing SDS-PAGE wulf-dirk.leuschner at tuebingen.mpg.de
help:clone a large fragment wei yongjie
HELP!! Wizard genomic DNA isolation xx yy
HELP! Wizard genomic purification kit xx yy
Best method for freezing tissue for RT-PCR z
protein purification z
EMSA optimization? z
tRNA isolation zeleznik at bscr.uga.edu
subscribe methods zhangyl at PUBLIC.CC.JL.CN
find friends share interest in GRF zhangyl at PUBLIC.CC.JL.CN

Last message date: Sat Jan 31 23:36:35 EST 1998
Archived on: Sun Mar 6 21:38:11 EST 2005

Messages sorted by: [ thread ] [ subject ] [ date ] Index

This archive was generated by Pipermail 0.09 (Mailman edition).