Methods January 1998 Archives by date

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Starting: Thu Jan 1 04:46:56 EST 1998
Ending: Sat Jan 31 23:36:35 EST 1998
Messages: 709

• first time the first...   Cattaruzza at veg-physiol.med.uni-goettingen.de
• A Non high fidility 5.3K PCR?   Svetlov Vladimir
• (none)   Sven Schoeppner
• Spreadsheet invention   Dan Pedersen
• Low or No Background Cloning   Wilson
• CSL Company - HELP!!!   Krzysztof Wasowicz
• QuickHyb and Nonisotopic hybridization   Bernd Kazmierczak
• HELP!!!white bands on my autoradiogram!   D.L.Clarke
• NP40 (was Re: nucleus removal in Sf9 cells.)   Arthur Wohlwill
• detection of green fluorescent protein   Yanghm
• agarose gel electrophoresis conditions   Joseph C. Bagshaw
• Will Dpn I cut half-methylated DNA?   Wolfgang Schechinger
• Help optimize electroporation   Jayati Kesavan
• JM108   Joan Boyce
• Qiagen's California address   J. Boyce
• Thermostable RT   J. Boyce
• Bird sexing using feather as the source of DNA   Gizbab
• for testing   s952003 at MAILSERV.CUHK.EDU.HK
• CSL Company - HELP!!!   Dianne Emslie
• subscribe methods   zhangyl at PUBLIC.CC.JL.CN
• library screening for secreted proteins   Dr GC Oliveira
• Help: PAGE gels cracking   lee.285 at osu.edu
• No plasmid form transformed BL21(DE3)   Ke-Xue Huang
• find friends share interest in GRF   zhangyl at PUBLIC.CC.JL.CN
• CSL Company - HELP!!!   thu nhat thi nguyen
• help   池野　聡一
• Help: PAGE gels cracking   Zophonias O. Jonsson
• silence a gene by insertion of an antibiotic casette   Marina Verkhovskaya
• Low or No Background Cloning   qin ling
• HELP: Protein overexpression using pTRXFus   sathish
• Which is better: RPA or RT-PCR   Harry Witchel
• prediction of protein-protein contact sites   Carsten Hohoff
• problems in cell culture   zhang jiping
• storage of westerns   John Richburg
• NP40 (was Re: nucleus removal in Sf9 cells.)   David L. Haviland, Ph.D.
• HELP!!!white bands on my autoradiogram!   kong
• NP40 (was Re: nucleus removal in Sf9 cells.)   Ian A. York
• storage of westerns   Brian S. Thoma
• EtBr vs 32P in RT-PCR   Ross Feldberg
• Mouse ras and bcl2 primers   snyderrf
• silence a gene by insertion of an antibiotic cassette   R.D. Haigh
• OPTIMIZING RECOMBINATION TO OBTAIN STABLES   paul gold
• Ammonium Acetate 'going bad?'   Marieke R. Koedood Zhao
• Genomic library fused to HIS3?   aplummer at dax-atm.rz.uni-frankfurt.de
• Source for digitonin?   Ian A. York
• SEAP assay   Mike Smith
• prediction of protein-protein contact sites   Vladimir Svetlov
• Will Dpn I cut half-methylated DNA?   Richard J. Dudley
• Detection of early transcripts? References required?   D.L.Clarke
• Postdoc Position   Hoyun_Lee at cancercare.on.ca
• Best method for freezing tissue for RT-PCR   Margaret Koziel MD
• Pre-cast SDS-PAGE gels for Hoeffer   Jeffrey Lin
• Help optimize electroporation   Dima Klenchin
• (none)   Dan Pelah
• Double insert ligation   T.J. Young
• Double insert ligation   Ned Mantei
• Double insert ligation   David C. Logan
• Best method for freezing tissue for RT-PCR   z
• non-radioactive detection kit   Dan Pelah
• B-galactosidase inhibitor   Dan Johnson
• non-radioactive detection kit   Bill McKendree
• No plasmid form transformed BL21(DE3)   Kevin A. Morano
• Endotoxin removal   James
• Best cell line for expression cloning but not COS-7 ?   Christophe Grundschober
• SDSPAGE Problems   Fergus Doherty
• Best cell line for expression cloning but not COS-7 c   Ian A. York
• storage of westerns   Marisol Rodriguez Pena
• qe: re-use of electroporation cuvettes   Reinhard Windoffer
• Which is better: RPA or RT-PCR   Marisol Rodriguez Pena
• <no subject>   Marisol Rodriguez Pena
• Best cell line for expression cloning but not COS-7 ?   Dom Spinella
• Fission Yeast protocols on WWWeb   Hochstenbach F
• re-use of electroporation cuvettes   R.D. Haigh
• FasL mono, di-, and trimers   John Richburg
• Plasmid/help   Manash
• Best cell line for expression cloning but not COS-7 c   Ian A. York
• Best cell line for expression cloning but not COS-7 c   Dom Spinella
• Best cell line for expression cloning but not COS-7 c   Ian A. York
• Best cell line for expression cloning but not COS-7 c   Dom Spinella
• New Product for Gene Regulation Studies - FREE!   Simon Sims
• library screening for secreted proteins   Peter Wang
• SDSPAGE Problems   Dr E. Buxbaum
• Endotoxin removal   Mike Amstutz
• Best cell line for expression cloning but not COS-7 ?   Ned Mantei
• Partners wanted for dev't of new combinatorial ch'try method   Sipke Wadman
• quantif. of DNA/RNA copy nbrs for Q-PCR.   Ladriere
• Ustilago protocols   jan.morren at bio.kuleuven.ac.be
• site-specific phosphorylation   Joel Baguet
• storage of westerns   Frank O. Fackelmayer
• S1 RNA Analysis   January Weiner
• storage of westerns   Martin Offterdinger
• Ni-NTA preparation   m griep
• Glycerol and Tm   vilimf01 at mcrcr6.med.nyu.edu
• Easiest Method of Synchronizing CHO Cells   paul gold
• Theoretical question: Is recombination efficiency cell cycle dependent?   paul gold
• image analysers   Jo Daniels
• CMF hanks buffer   Mathias DREXLER
• Double insert ligation   Victor Levenson
• in vivo chemical cross-linkers   Joel Baguet
• qe: re-use of electroporation cuvettes   Harold E Smith
• agarose gel electrophoresis conditions   Tim Salmon
• [Q]help-TA cloning after PCR   601
• RPA or RT-PCR   HRIND
• T7 RNA polymerase phage?   Brad Pietz
• Source for digitonin?   Nicole Rapior
• 18s rRNA probe   Z.Huang
• CMF hanks buffer   Paul R. Rohde
• Electrophoresis, TLC, Image analysi   aab at IX.NETCOM.COM
• Q:QuickChange and deletions   Hadi
• ### $35 FOR $300 IN FREE FAST FOOD AND $$$ ###   freefastfood at HOTMAIL.COM
• Easiest Method of Synchronizing CHO Cells   Martin Offterdinger
• Endotoxin removal   Administrador de iihema.fmed.uba.ar
• Immunoprecipitation   Marisol Rodriguez Pena
• Endotoxin Removal   Wolfgang Schechinger
• protein purification   K.G.Spink
• protein purification   z
• Endotoxin Removal   Harry Witchel
• protein-protein interaction   Lion
• Post-DD problem   Maaria Roschier
• plant expression vector   Bill McKendree
• Update on Used Lab Equipment Site   James J. Campanella
• DNA proofreading and growth   Matt Thomas
• ELISA plate readers   Susanne Kjemtrup
• 18s rRNA probe   Christopher Zhao
• Plasmid Isolation from Yeast   byudkin at bioch.ox.ak.uk
• Yeast Plasmid Isolation   mabenson at technologist.com
• [Q]help-TA cloning after PCR   Dom Spinella
• RPA or RT-PCR   Caroline Szymeczek-Seay
• Tet-ON vector(s)   Patrizia Dell'Era
• T7 RNA polymerase phage?   Peter Wang
• Genomic RDA?   Mike Holloway
• lectin for O-linked sugars   RAY MCGUIRK (BIOCHEMISTRY) PG
• Q:QuickChange and deletions   Vladimir Svetlov
• Yeast Plasmid Isolation   Vladimir Svetlov
• Plasmid Isolation from Yeast   Vladimir Svetlov
• DD-RT-PCR   Brian
• storage of westerns   Luis Alvarez
• Ultra-low temp. freezer   Hoyun_Lee at cancercare.on.ca
• SeaLite Science AquaLite   Wilkinson, Deborah
• DIG In Situs   Asim
• Metabolic labeling of human monocytes   Blair R. Renshaw
• HELP!!!white bands on my autoradiogram!   LouPass
• Agarose gel melts at room temp?   Alex Abbas
• [Q]help-TA cloning after PCR   Jiqing Peng
• DD-RT-PCR   Lawrence Chen
• Ammonium Acetate 'going bad?'   Alex Dobrovic
• Ammonium Acetate 'going bad?'   Mark Dowton
• Any experience with TOPO PCR cloning kit?   Michael Lassner
• Post-DD problem   Mart Speek
• DNA software for Linux   January Weiner
• ddisplay   Mr. J Gaughan
• Any experience with TOPO PCR cloning kit?   Yoram Gerchman
• Plasmid Isolation from Yeast   Matt Benson
• Yeast Plasmid Isolation   Richard Grant
• T7 RNA polymerase phage?   is.bbsrc.ac.uk
• DNA software for Linux   Keith James
• Any experience with TOPO PCR cloning kit?   Bill McKendree
• T7 RNA polymerase phage?   Wolfgang Resch
• software for searching repeat sequence   Ke-Xue Huang
• in vivo chemical cross-linkers   Souren Mkrtchian
• Ultra-low temp. freezer   Pamela Norton
• pET-vector   Christian Ebeling
• New Section in AgBiotech Reporter   Andrew R. Apel
• Need PFGE apparatus suppliers   David Neece
• Dye terminator composition????   fwhyde at ibm.net
• DIG In Situs   Paul C. Grimm
• Need PFGE apparatus suppliers   Tony Sanchez
• invitro translation   Xiao-Hua Xue
• DD-RT-PCR   D.L.Clarke
• EMSA optimization?   Mirkka Herranen
• Refridgerated Microcentrifuges.   Marie Estock
• GT repeat characterization   agabiti at master.cci.unibs.it
• lectin for O-linked sugars   Nicole Rapior
• polyclar-AT   Bruce A. Caldwell
• Agarose gel melts at room temp   Cres Bookstein
• ddisplay   Cres Bookstein
• Refridgerated Microcentrifuges. => buy rotor (Beckman JA-18.1) for your existing centrifuge instead   Dima Klenchin
• No plasmid from trasformed BL21(DE3)   Ke-Xue Huang
• Ph.D. student position/Sweden (plant mol. biol.)   Leszek Andrzej Kleczkowski
• [Q]help-TA cloning after PCR   user
• help:clone a large fragment   wei yongjie
• (none)   Mr. Daniel Cadolle
• Help a student with science fair   sean
• No plasmid from trasformed BL21(DE3)   Cornelius Krasel
• looking for gamma glutamyl transferase antibody   Max Davidson
• plant sulfur metabolism   Oliver Berkowitz
• T7 RNA polymerase phage?   Christian Ebeling
• In situ hybridization in pathology   Andrew Scott
• No plasmid from trasformed BL21(DE3)   John R. Thompson
• EMSA optimization?   P.J. Shaw
• help:clone a large fragment   ChemicalSuperFreak
• In situ hybridization in pathology   ChemicalSuperFreak
• Expressing the cpp32 in yeast nucleus   Dooyeon Kim
• non-radioactive RPA   Clair Lee
• DD-RT-PCR   Paul R. Rohde
• Chr 12 markers Help   s.pritchard
• IEF   Robert Woodward
• EMSA optimization?   z
• Chr 12 markers help   Robert B. Chadwick
• pH-meter   Michael J. Tuvim
• Any experience with TOPO PCR cloning kit?   Richard J. Dudley
• touchdown PCR using pfu polymerase   Jun-Ichi Aikawa
• gfp-neo fusion   Matthew D. Beasley
• Q:QuickChange and deletions   CCoburn
• Expressing the cpp32 in yeast nucleus   Dooyeon Kim
• MBP fusion protein does not bind to amylose?   ZhongLingChai
• FIAU -- Looking for source of FIAU   Don Wang
• Vertical electrophoresis unit   Anton Posch
• Double insert ligation   Koen De Smet
• Double insert ligation   Richard Grant
• Melting temp formula for RNA:RNA   Andy Phillips
• Melting temp formula for RNA:RNA   Andy Phillips
• DNA from serum   Jean-Pierre de Villartay
• Tm of RNA/RNA hybrids   Andy Phillips
• FIAU -- Looking for source of FIAU (Ganciclovir)   Wolfgang Schechinger
• GST fusion protein solubility   atanasio
• protein-DNA coimmunoprecipitation   Flore BELLEFONTAINE
• Stable cell transfection   James
• Melting temp formula for RNA:RNA (sorry)   Andy Phillips
• Tm of RNA/RNA hybrids   Mark Asnicar
• HELP!! Wizard genomic DNA isolation   xx yy
• GT repeat characterization   GR Taylor PhD MRCPath
• Stable cell transfection   Ian A. York
• [2hybrid] HF7c [URA-] ?   Pierre Lindenbaum
• GST fusion protein solubility   Randall C Willis
• GST fusion protein solubility   Keith Jolley
• help:clone a large fragment   Christophe Jacob
• invitro translation   A.Habel
• help:clone a large fragment   Andy Phillips
• New SDS-PAGE / Western Blotting Review   Shaun D. Black
• SDS-PAGE gel-shift to look at protein phosphorylation   John Pawlowski
• [Q]help-TA cloning after PCR   Dr. Duncan Clark
• Amplification of cDNA inserts from freeze-thawed phage   Jorge Vasquez-Kool
• qe: re-use of electroporation cuvettes   Rob Kirkpatrick
• help:clone a large fragment   Daniel Schlieper
• Salmonella gDNA library   Ann Eakin
• Best cell line for expression cloning but not COS-7 c   David de Graaf
• genomic PCR help   Jennifer Pan
• Best cell line for expression cloning but not COS-7 c   Dom Spinella
• site directed mutagenesis   ANNE GEORGE
• GST fusion protein solubility   Arnoud van Vliet
• purification of CD3, CD14 and CD19 cells   Sylvie Provost
• Help!!>> DNA Replacement   Hynumin Kim
• genomic PCR help   John Ladasky
• fabrication of nylon membrane discontinued   Edith Duerbaum
• Stable cell transfection   Kiley R. Prilliman
• site directed mutagenesis   Paul R. Rohde
• site directed mutagenesis   Paul R. Rohde
• ECACC   un691cs at genius.embnet.dkfz-heidelberg.de
• proline not by HPLC?   Kerstin Waigand
• Amplification of cDNA inserts from freeze-thawed phage   Dr. Duncan Clark
• elisa reader filter needed ANY HINTS ? / SLT lab instr.   T. Lenhard
• GST fusion protein solubility   Koen De Smet
• YEP357   Lestrate Pascal
• Amplification of cDNA inserts from freeze-thawed phage   Frank O. Fackelmayer
• elisa reader filter needed ANY HINTS ? / SLT lab instr.   Erich Lederer
• unsubscribe   Seok-min Yoon
• DNA labeling with fluorescein   Lestrate Pascal
• LA-PCR problems   Parent, Eric
• GEX-GST induction   Pierre Lindenbaum
• site directed mutagenesis   Lestrate Pascal
• best way to isolate bands from gels   Bike&Linux
• unsubscribe   beatrice.seguin at utoronto.ca
• Best cell line for expression cloning but not COS-7 c   ronlab
• Q: EST Databases other than GDB?   Wolfgang Schechinger
• site directed mutagenesis   Richard J. Dudley
• site directed mutagenesis   Vladimir Svetlov
• best way to isolate bands from gels   Vladimir Svetlov
• Address for making nucleotides   C.L.L.
• Q: EST Databases other than GDB?   Dom Spinella
• Histochemical techniques   Michael William Walsh
• Best cell line for expression cloning but not COS-7 c   David de Graaf
• PCR gradient thermocyclers   Frank Struyf
• SDS-PAGE gel-shift to look at protein phosphorylation   Bob Steinberg
• Poliovirus temperature stability?   David N. Levy
• ABI Linkage Mapping Set 2?   Robert B. Chadwick
• best way to isolate bands from gels   Ed Castro
• DNA-Protein Cross Linking Methods   Sudhanshu Dole
• best way to isolate bands from gels   Wolfgang Schechinger
• GST fusion protein solubility   Frederik Boernke
• cleavable tags   Cheryl L. Richards
• New Stratagene Select cII Kit-details?   fwhyde at ibm.net
• best way to isolate bands from gels   Wolfgang Schechinger
• IEF   Stephen Marek
• DNA labeling with fluorescein   Arnoud van Vliet
• PCR gradient thermocyclers   WSchick
• Custom service of scrrening of mouse genomic BAC library   Jun-Ichi Aikawa
• PCR gradient thermocyclers   Kiley R. Prilliman
• GAL-lacZ plasmid for yeast?   Justinian
• LA-PCR problems   John Ladasky
• textbooks of BAC and PAC genomic library   Jun-Ichi Aikawa
• help   JIANG SHOU/MICROBIOLOGY/IMMUNOLOGY
• Poliovirus temperature stability?   Karl Fischer
• HELP! Wizard genomic purification kit   xx yy
• elisa reader filter needed ANY HINTS ? / SLT lab instr.   Guy Hermans
• SDS-PAGE gel-shift to look at protein phosphorylation   Martin Offterdinger
• request for join   yanli wang
• What's the difference between homogenisation and sonication?   Chew Sek Jin
• Hifax   Wong Yau Ming
• help please...I need to buy equipment..and I dont know what to choose..   Lars Thomsen
• mouse beta-actin   wiebke hallensleben
• dNTP problems   TBott53
• DNA from serum   Ulrich Maier
• Protein-protein   ERNESTO OVIDEO ORTA
• What do you think of this CIP   Pierre Lindenbaum
• GST fusion protein solubility   Koen De Smet
• PCR gradient thermocyclers   Koen De Smet
• Gangliosides - Bachem?   Erik A.J. Miljan
• best way to isolate bands from gels   ChemicalSuperFreak
• help please...I need to buy equipment..and I dont know what to choose..   ChemicalSuperFreak
• BESS T-Scan Kit for Mutation Detection????   fwhyde at ibm.net
• BESS T-Scan Kit for Mutation Detection????   GR Taylor PhD MRCPath
• site directed mutagenesis   Wolfgang Schechinger
• SDS-PAGE gel shift to look at protein phosphorylation   Tim Salmon
• Best cell line for expression cloning but not COS-7 c   Wolfgang Schechinger
• HELP! Wizard genomic purification kit   Hiranya Roychowdhury
• [2hybrid] HF7c [URA-] ?   Ralf Weigel
• Q: Choccolate-like smelling bugs?   Wolfgang Schechinger
• help: genomic DNA extraction from tissue   Z.Huang
• HELP! Wizard genomic purification kit   Jennifer Philhower
• Sperm and RNA isolation   Rentia
• dNTP problems -Reply   Kathy Mangold
• Low-protease strain of B. subtilis   Arul Jayaraman
• End conversion of PCR products   craig garen
• Please help   JIANG SHOU/MICROBIOLOGY/IMMUNOLOGY
• Who makes MACS columns?   David N. Levy
• Q:genomic DNA extraction   AKi Hoji
• PVPP   Vijay Gadkar
• Q: Choccolate-like smelling bugs?   Roney Graf
• Help w/ Ni column protein purification   Keith
• Bio-Rad's gene gun tubing coating   bezstarosti at bc1.fgg.eur.nl
• best way to isolate bands from gels   Michael Rosemann
• ECACC   Michael Rosemann
• apo-protein of cyt c, isolation protocol...   ckmsl at uohyd.ernet.in
• precipitation of very small DNA fragments   Frank O. Fackelmayer
• Who makes MACS columns?   Darren A. Natale
• Q: Choccolate-like smelling bugs?   Martin Collinson
• pH of Tris   Martin Collinson
• Calibration of fura-2   Dr. K. Kluge
• help: genomic DNA extraction from tissue   s.pritchard
• Help w/ Ni column protein purification   Darren A. Natale
• Q:genomic DNA extraction   s.pritchard
• Own Your Own Power Company!!   powermlm1 at HOTMAIL.COM
• Help w/ Ni column protein purification   Vladimir Svetlov
• Help w/ Ni column protein purification   Keith Jolley
• Best cell line for expression cloning but not COS-7 c   ronlab
• help: genomic DNA extraction from tissue   Dr. Duncan Clark
• Help w/ Ni column protein purification   Dana Nojima (CBN)
• Q:Transfecting breast cancer cell lines?   jo Morris
• PVPP   Hiranya Roychowdhury
• STABILITY of solubilization buffer   Rocky Baker
• precipitation of very small DNA fragments   Ransom Hill Bioscience
• Protocol for Hydroxy Radical DNA-Footprints   Marco Gymnopoulos
• antibody to trpE gene product?   Regina Shaw
• Taq Extender - What is it?   bbeitzel at biomail.ucsd.edu
• DNA structure and PFGE   Edgar Valencia
• STABILITY of solubilization buffer   Hiranya Roychowdhury
• End conversion of PCR products   Hiranya Roychowdhury
• STABILITY of solubilization buffer   Randall C Willis
• elisa reader filter needed ANY HINTS ? / SLT lab instr.   Bob Kays
• Magnetic Carriers Meeting - CALL FOR ABSTRACTS   Urs Hafeli
• Mac molbio software recommendations?   Charles Brenner
• Dye terminator composition????   Wayne M. Barnes
• SDS-PAGE gel-shift to look at protein phosphorylation   Brian Hollander
• vector replicon   John E. Butler
• Help w/ Ni column protein purification   Dima Klenchin
• Help w/ Ni column protein purification   Dima Klenchin
• help   Seok-min Yoon
• RNA isolation from tissue perfused w/UW solution   Randy Haun
• RPA--suggestions about which are the best kits.   AO
• Correct sizing of alleles after silver staining of polyacrylamide gels   E.C.WATSON at lboro.ac.uk
• µM -- please check my calculation   Lawrence Chen
• Q:Transfecting breast cancer cell lines?   delios97 at hotmail.com
• BM's DNA binding protein purification kit.   Sudhanshu Dole
• BLASTn vs. BLASTp   Colby Zaph
• Boehringer M.'s DNA binding protein purification kit.   Sudhanshu Dole
• best way to extract DNA from gel   QGUO1
• Yeast Plasmid prep   QGUO1
• RNA isolation from tissue perfused w/UW solution   Paul R. Rohde
• help: genomic DNA extraction from tissue   Alex Dobrovic
• (none)   Mr. Daniel Cadolle
• Losing the plasmid   Mary P. Remington
• Losing the plasmid   Arnoud van Vliet
• need RNA marker and Nytran.   hongxiang hui
• Patch-clamping transfected mammalian cell lines?   Harry Witchel
• Hybridization enhancement   Karl Fischer
• What is the best kit for mRNA purification?   Jun-Ichi Aikawa
• Histochemical techniques   Krzysztof Wasowicz
• Who makes MACS columns?   Sebastian Bunka
• best 2 hybrid system   Dimitri Harmegnies
• ELISA and Alkaline phosphatase-DNA probes: How to calculate signal:noise?   Cytocell Ltd.
• Alkaline phosphatase-DNA probes: How to calculate signal:noise?   Cytocell Ltd.
• plastic-binding phage   Borg Collective
• Q:QuickChange and deletions   Vladimir Svetlov
• plastic-binding phage   Dom Spinella
• Electrotransfer of high MW proteins   Mrta Grifman
• 1D-PAGE software: free/shareware??   ecastro at eucmax.sim.ucm.es
• Hybridization enhancement   Mr. T. Weissensteiner
• best 2 hybrid system   Frederik Boernke
• Electrotransfer of high MW proteins   PbZep
• magnetic beads   markku Aalto
• Test   Bubs
• Lambda zap V lambda dash   Richard Johnson
• DNA structure and PFGE   Edgar Valencia
• Lambda Zap v/s Dash   Ramaswamy K. Iyer
• pYX212 plasmid sequence ?   Pierre Lindenbaum
• Q:Transfecting breast cancer cell lines?   Martin Offterdinger
• Electrotransfer of high MW proteins   Takashi Umeyama
• Help w/ Ni column protein purification   Koen De Smet
• Histochemical techniques   Tim Fitzmaurice
• 1D-PAGE software: free/shareware??   Jay Miller
• Info   immun
• (none)   immun
• ER/cytoplasmic prep   Michael Daws
• Looking for Rho small G proteins   Wolfgang Schechinger
• Q: Choccolate-like smelling bugs?   Phillip San Miguel
• Help w/ Ni column protein purification   Lars Komorowski
• Patch-clamping transfected mammalian cell lines?   Lars Thomsen
• pMOB plasmid sequence   Wood Lab
• DNA structure and PFGE   Phillip San Miguel
• Help w/ Ni column protein purification   Michael Hodges
• Stable cell transfection   Hendrie Kain
• Looking for Rho small G proteins   Ole Gjoerup
• Predicting Coiled Coils from Protein Sequences   Alex Knight
• TRINUCLEOTIDE REPEATS   James W. Larkin
• Yeast strains question   Zé Guerra
• Electrotransfer of high MW proteins   Shaun D. Black
• Isolation of cell membranes from tisue culture cells.   Judith Blasser F-H
• probe labeling   Michael Flores
• Redissolvinging lyophilised DNA problems   Alex Dobrovic
• Custom Transfection   Michael Rist
• E. coli. strain B834 commercially available?   Zhonglin Chai
• Western Blot Problem   Ramaswamy K. Iyer
• Help: Density of a typical bacterial cell ( eg. E. Coli )   Bin Lin
• Protinase K long-term storage, again   AKi Hoji
• RNA isolation from Xmas tree   kp at everx.szbk.u-szeged.hu
• oligo dT column too slow   Kent S. Boles
• Tm and Hybridization temperature   CVRL
• vacuole transport in pombe   T.J. Young
• 96 well Coli cultures   Michael O'Donohue
• software for finding restriction sites   C.S. Wilding
• BLASTn vs. BLASTp   Andy Phillips
• T-DNA plasmid required urgently   Fornavn Etternavn
• Tm and Hybridization temperature   Andy Phillips
• Protein purification using guanidinum hydrochloride   James
• software for finding restriction sites   Wolfgang Schechinger
• test- please ignore   john brennand
• Protein purification using guanidinum hydrochloride   Randall C Willis
• RNA extraction from liver   Joel Baguet
• Help: Density of a typical bacterial cell ( eg. E. Coli )   Pascal Mertens
• normalized cDNA libraries   mdraper at opal.tufts.edu
• Electrotransfer of high MW proteins   Joel Baguet
• Commercial source of full length cDNA clones.   Donald Dunbar
• oligo dT column too slow   David L. Haviland, Ph.D.
• Genomic library   Z. Zhao
• ER / cytoplasmic fraction preparation   Michael Daws
• Electrotransfer of high MW proteins   Wolfgang Schechinger
• LA PCR   Parent, Eric
• Ni-NTA again   Lars Komorowski
• Electrotransfer of high MW proteins   Stephan
• BLASTn vs. BLASTp   Frederik Boernke
• Software for Sequencing Projects?   Stephan
• sex-specific labelling of cells?   Nicholas J. Gibson
• pH of Tris   Lou Hom
• probe labeling   as-sm at cyberus.ca
• in vitro transcription   ryyukhan at FACSTAFF.WISC.EDU
• precipitation of very small DNA fragments   CCoburn
• Lambda zap V lambda dash   Joseph C. Bagshaw
• southern enigma   William Sun
• Southern Enigma   William Sun
• SSCP   Gregory Buzard
• Commerical Bacculovirus Service??   Scott Andrews
• Phaseolus vulgaris genomic or leaf cDNA library   mjohnson at biology.as.ua.edu
• References: Electrotransfer of high MW proteins   Stephan
• probe labeling   Lab
• Reprobing of western blot   Frederik Boernke
• Vectors for testing promoters in plants   Steve Rice
• SSCP   Beth Wapelhorst
• Q:QuickChange and deletions and native Pfu and cloned ....   hadi
• probe labeling   Beth Wapelhorst
• Expression of toxic protein   Wan Seok Yang
• software for finding restriction sites   eizema at bc1.fgg.eur.nl
• Reprobing of western blot   eizema at bc1.fgg.eur.nl
• rat growth factor desperately wanted   Peter Wollenberg
• Dye for Sephadex column   Pete's Person
• low copy origin for expression plasmid   Tosso Leeb
• Software for Sequencing Projects?   Crepineau florent
• C-term labeling of a peptide ?   Josef P. Magyar
• southern enigma   Mart Speek
• in vitro transcription   Warren Hirst
• RNA extraction from liver   Warren Hirst
• Alternative Polyadnylation sites   Carmel Curtis
• in vitro transcription   Martin Offterdinger
• software for finding restriction sites   Rork D. Kuick
• E. coli. strain B834 commercially available?   Jakob Begun
• southern enigma   Chris Boyd
• ECL   Sandra Schneider
• Dye for Sephadex column   Dom Spinella
• C-term labeling of a peptide ?   Dom Spinella
• software for finding restriction sites   deepakk at mailcity.com
• genotyping SCID and Nu/Nu mice   lr50916 at glaxowellcome.com
• in vitro transcription   Heinz J. Schaefers
• beta actin   Dr. Cora Weigert
• southern enigma   Karl Fischer
• HELLO   jeskritt
• software for finding restriction sites   lr50916 at glaxowellcome.com
• software for finding restriction sites   Dr. Dirk Seegert
• in vitro transcription   brett
• Southern Enigma   David Winterbourne
• T-DNA plasmid required urgently   J. Hendriks
• Western Blot Problem   Daniela Gadotti
• RNA extraction from liver   J. Hendriks
• DNA invitro-assay   Nikolai Klein
• tRNA isolation   zeleznik at bscr.uga.edu
• 2-D PAGE training - attend a 3 days course in Cologne, Germany   Anton Posch
• In situ ...   Timur Yarovinsky
• PCR cloning in phage   Timur Yarovinsky
• DNA Isolation   Guy Tel-Zur
• DNA Isolation   Arnoud van Vliet
• Mac molbio software recommendations?   Jeff Huckaby
• PCR ribotyping with 16S-23S IGS   Kirsty Townsend
• 1D-PAGE software: free/shareware??   Jeff Huckaby
• Software for Sequencing Projects?   Jeff Huckaby
• CD13 antibodies   Michael J. Herron
• how do I quit methods   beatrice.seguin at utoronto.ca
• detecting RNA AND protein from same gel/filter   bongo
• Help:Mycoplasm contamination in Vaccinia soup   AKi Hoji
• southern enigma   Mart Speek
• Help: How to reduce autofluorescence in brain sections?   Ulrike Gausmann
• Electrotransfer of high MW proteins   Tom Vink
• Oligo hybridization.   Peter Nilsson
• [Q] Anti-p53(Bovine) antibody info's   Martina Plass
• protein precipitation   RAY MCGUIRK (BIOCHEMISTRY) PG
• protein precipitation   Ian McFarlane
• [Q]help-TA cloning after PCR   Marina Verkhovskaya
• Small protein Western Analysis   A P Smith
• Excerpt from AgBiotech Reporter - Call for Submissions   Andrew R. Apel
• [Q]help-TA cloning after PCR   DOCTORHIM
• NdeI   Richard P. Grant
• NdeI   Scott Andrews
• Cloned beta-galactosidase from Strep. pneumonia   HMiller774
• Expression of toxic protein   ijiwaru at dcn.davis.ca.us
• A minimal medium for S. aureus RN4220   Brian Smith
• OK to send e-mail?   owl at OWLSEYE.COM
• Is a 15 na oligo ok for colony hybridization?   REWHLAB
• NdeI   Vladimir Svetlov
• NdeI   Ian A. York
• Problems with restriction digests   Hopkins, Charlotte
• Expression of toxic protein   DOCTORHIM
• Expression of toxic protein   Lyle Najita
• Many questions   Jürgen Häußler
• Is a 15 na oligo ok for colony hybridization?   Timaoxin
• BIOSCI/bionet miniFAQ & Fundraiser   BIOSCI Administrator
• Commerical Bacculovirus Service??   Scott Andrews
• Differential display problems   John Landers
• normalized cDNA libraries   Harold E Smith
• coating microscope coverslips with streptavidin   Jonas Korlach
• Small protein Western Analysis   Per Mygind
• Small protein Western Analysis   Christian Radauer
• Home Bussiness   esikes at ANET-DFW.COM
• NdeI   Michael O'Donohue
• silver stain; sensitivity RNA vs. protein   Juergen Brosius
• [Q]: RT-PCR 1st strand kit   Sebastian Bunka
• sequencing   Neil Butt
• tissue culture solutions   Shlomo Amir
• Genuine **HOME-BASED** Employment Offer:   up_yors11 at NETIMATION.COM
• >DD-PCR problem   Mr. T. Weissensteiner
• Gene Hunter company's add.   VASANTHARAJAN JANAKIRAMAN
• Is a 15 na oligo ok for colony hybridization?   Tom Chappell
• Gene Hunter company's add.   Glenn Knight
• A minimal medium for S. aureus RN4220   Ralf Oltmanns
• in vitro transcription   Mac Twocx
• Your experience with RDA?   Mike Holloway
• total protein extraction for FPLC   Heinz J. Schaefers
• Commercial source of full length cDNA clones.   Darren Tyson
• New World Monkey genomic library   Juergen Brosius
• NdeI   Vladimir Svetlov
• Lawsuit   admin at alt.misc.news.legal
• Commerical Bacculovirus Service??   Sam Lee
• ctab hyb enhancements   elader at ambion.com
• RNA from christmas trees   elader at ambion.com
• oligo dT column too slow   elader at ambion.com
• Hyaluronic acid:Extraction, Purification, Sterilization   Thanit Pewnim
• Pharmaceutical XRD Data plus warning for International Quant Analysis Round Robin participants   Lachlan Cranswick
• Expression of toxic protein   Thomas
• total protein extraction for FPLC   Cornelius Krasel
• Help! Antibodies!   Mr. G. Morley
• Problems with restriction digests   Michael Rosemann
• Cloned beta-galactosidase from Strep. pneumonia   DOCTORHIM
• C-term his6 tag   Harold J. Drabkin
• tRNA isolation   Harold J. Drabkin
• Oligo hybridization.   Harold J. Drabkin
• Great News: Bioinformation Search Center Version 3.0 !   Mousheng Xu
• NdeI   C.L.L.
• E. coli Cu++ Sensitivity?   Rick Davis
• total protein extraction for FPLC   John A. Newitt
• Reprobing of western blot   Darren Tyson
• Problems with restriction digests   Richard L
• Question on Streptococcus pneumoniae   Richard L
• Gene Hunter company's add.   Randy Haun
• ? E.Coli competent greater than 10 +E 8 without electroporation   reber at uni-bonn.de
• conditions for DNA binding   cll
• Epitope Mapping Software [Q]   Michael Oda
• ? E.Coli competent greater than 10 +E 8 without electroporation   Tim Barnett
• ECACC   Axel Schumacher
• storage of bacterial plates   Tulene McCabe
• Problems with restriction digests   Keith Rand
• Background plaques when screening Lambda ZAP II genomic libraries with DNA probe   Michael
• Disposable Oak Ridge tubes   Tom Glenn
• AFLPs using S35?   Pete's Person
• Commercial source for SV40 large T-ag?   René Assenberg
• help   Christian Petzelt
• Is Your Web Site A Secret?   owl at owlseye.com
• [Q]help-TA cloning after PCR   Roderic Fuerst
• ctab hyb enhancement   Mr. T. Weissensteiner
• need bacteria TOP10/P3 or pcDNAI-Amp   Said Hashemolhosseini
• ECACC   Fraser Dr N J
• ECACC   Fraser Dr N J
• JM110   Per Mygind
• CAT antibody   kev
• E. coli Cu++ Sensitivity?   Frank O. Fackelmayer
• protein precipitation   Jeffrey A. Cohlberg
• 6His protein   Henri M.H. Spronk
• JM110   Richard J. Dudley
• Invitro trascription vector with poly AA?   Richard J. Dudley
• How to separate bulged from perfect DNA duplexes?   Malcolm White
• Calcium Binding Proteins Symposium in Germany   Ralf Spenneberg
• Separation of bulged from perfect duplex DNAs   Malcolm White
• protein precipitation   Peter Pediaditakis
• OK to send e-mail?   Andrew Leech
• Gen bank   tlibert
• cloning woes   Robot-Boy
• mtDNA invertebrates universal primers   Parent, Eric
• Epitope Mapping Software [Q]   Tom Vink
• HPLC for Protein Separation and Identification   yuanxiang Zhou
• software for finding restriction sites   paul lacy
• protein recovery   Anwar A. Khan
• Reprobing of western blot   snyderrf
• Invitro trascription vector with poly AA?   Hallgeir Bergum
• 2hybrid:Expression level of bait vs prey   Frederik Boernke
• stripping RNA probes   Andrew Warkman
• mtDNA invertebrates universal primers   HaroldB786
• Is Your Web Site A Secret?   owl at OWLSEYE.COM
• intein system   Hans-Helmut Niller
• bandshift in agarose gel   Markus Zettl
• HPLC for Protein Separation and Identification   Advanced Biotechnologies
• Serum Albumin on Platinum   Joachim Steiner
• 3'end mapping   patcharaporn deshnium
• Chlamydia Pneumoniae   Per Mygind
• 3'end mapping   lr50916 at glaxowellcome.com
• Transfection problems   lr50916 at glaxowellcome.com
• Chlamydia Pneumoniae   J.M. Ossewaarde
• genotyping SCID and Nu/Nu mice   Jorg Kirberg
• Volatile buffer pH7.5?   Ian McFarlane
• Follow-up : hybridization enhancement   Karl Fischer
• Question on Streptococcus pneumoniae   Ulrich Maier
• Addendum: Hybridization enhancement   Karl Fischer
• SPA (Single Primer Amplification)   Yves Bertheau
• Km values handbook?   Georg Kroeger
• Km values handbook?   Jeffrey A. Cohlberg
• 6His protein   Vladimir Svetlov
• differential display equipment   Michael C. Gorry
• Transfection problems   Rentia
• Expression of toxic protein   Chen Ho An
• mtDNA invertebrates universal primers   Black, Michael
• differential display equipmentPS   Dom Spinella
• Transfection problems   Dom Spinella
• need BL21A(DE3) strain of E. coli   bipin k. dalmia
• intein system   Roland Saldanha
• genotyping SCID and Nu/Nu mice   David de Graaf
• Invitro trascription vector with poly AA?   Randy Haun
• genotyping SCID and Nu/Nu mice   Jorg Kirberg
• stripping RNA probes   Judi
• Q: cultivation of ES and feeder cells.   ronlab
• in vitro transcription   Darren Boehning
• genotyping SCID and Nu/Nu mice   David de Graaf
• Transfection problems   John Ladasky
• Inverse PCR -- degraded DNA ends?   John Ladasky
• Adenoviral Titer Protocols   R. Brooks Robey
• His-tag inhibits anti-myc-antibody   wulf-dirk.leuschner at tuebingen.mpg.de
• thin bands in non-reducing SDS-PAGE   wulf-dirk.leuschner at tuebingen.mpg.de
• His-tagged protein, urea & antibody production   g. lawrence
• His-tagged protein, urea & antibody production   Glenda Lawrence
• Chlamydia Pneumoniae   Ladriere
• E. coli Cu++ Sensitivity?   Lars Komorowski
• Volatile buffer pH7.5?   Laurent POULOUIN
• Que] about Conjugation?   Hyunmin Kim
• c-myc in plants   Jan Carette
• Volatile buffer pH7.5?   Martin Webb
• RNA standard for quantitative rt-pcr   Vidar
• DNA isolation from hair   John Korte
• What size tip for Sonifier?   Mary P. Remington
• His-tagged protein, urea & antibody production   Frederik Boernke
• Linear vs. supercoiled DNA in plasmid transfection   Jose E. Mejia
• What size tip for Sonifier?   DOCTORHIM at AOL.COM
• storage of bacterial plates   Marieke R. Koedood Zhao
• DNA isolation from hair   John Ladasky
• CV-1 genomic library   Richard Pelletier
• DD RT-PCR   fx.maquart
• What size tip for Sonifier?   WSchick at AOL.COM
• DNA isolation from hair   HaroldB786
• Better chelator of zinc than EDTA?   Damaged Goods
• blue crab cDNA library   marius brouwer
• Gibco RNAse mix??   Peta C. Bonham-Smith
• Better chelator of zinc than EDTA?   Arnoud van Vliet
• Better chelator of zinc than EDTA?   DOCTORHIM at AOL.COM
• Better chelator of zinc than EDTA?   Ashok Aiyar
• Gibco RNAse mix??   Bryan L. Ford
• DNA isolation from hair   ChemicalSuper Freak

Last message date: Sat Jan 31 23:36:35 EST 1998
Archived on: Sun Mar 6 21:38:11 EST 2005

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