How to seq. a single-primer PCR product

Brian Sydnor Burnes gt2837a at acmey.gatech.edu
Thu Jul 2 10:57:43 EST 1998


Hello fellow labrats!  I have PCR products generated from inverse PCR
primed off the inverted repeats of a Tn5 insertion.  Only one primer was
used, so the ends of the products are inverted repeats like the
transposon.  How can I directly sequence these products without getting
overlapping forward and reverse reads?  Sequence an asymetric digest?
Ligate (blunt) into a sequencing vector?  I appreciate any input; thanks.


--
Brian Sydnor Burnes
Georgia Institute of Technology, Atlanta Georgia, 30332
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