Bac-to-Bac system

Andreas Vogel vogela at uni-muenster.de
Fri Jul 3 03:49:12 EST 1998


Hi all,

I am going to start with baculovirus expression and decided to use the
Bac-to-Bac system from life technologies. They say you dont have to
waste your time with plaque purification because you can generate 100
percent recombinant virus DNA. 
But, are there really no difficulties with this system? Does anyone have
experience with it?

Thanks in advance for your help,
Andreas 

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Andreas Vogel                
Department of Biochemistry      
University of Münster       Tel: +49-251-8332128
Wilhelm-Klemm-Str. 2        Fax: +49-251-8333206
D-48149 Münster/Germany     E-mail: vogela at uni-muenster.de
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