"flash-freezing" denatured probes

sarah_petersen at yahoo.com sarah_petersen at yahoo.com
Sat Jul 4 13:01:31 EST 1998


Hi,

I often see the instruction to 'quickly cool' a denatured probe before adding
it to the hybridization buffer. If you're going -straight- from boiling to
the hyb bottle, is this neccessary? Is it bad for the probe to slowly cool to
the hyb temp? Any thoughts on this?

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