Bac-to-Bac system

Gregg Silk greggsilk at
Sun Jul 5 23:35:19 EST 1998

We had a long thread about this last month. It works pretty well, you generate
the recombinants in a special E. coli host (not insect cells), do selection,  
minipreps, and characterization THEN transfect insect cells.  A round of
screening might be needed to purify isolates. But it is much much faster AND
much cheaper than many alternatives.

Gregg W. Silk

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