Bac-to-Bac system

Gregg Silk greggsilk at aol.com
Sun Jul 5 23:35:19 EST 1998


We had a long thread about this last month. It works pretty well, you generate
the recombinants in a special E. coli host (not insect cells), do selection,  
minipreps, and characterization THEN transfect insect cells.  A round of
screening might be needed to purify isolates. But it is much much faster AND
much cheaper than many alternatives.

Gregg W. Silk



More information about the Methods mailing list