Background when using AP immunodetection system

ruckeri at ruckeri at
Sun Jul 5 23:37:18 EST 1998

I am doing Western blotting using a PVDF membrane and an amplified alkaline
phosphatase detection system.  I am getting high levels of background
staining and am wondering if it is because of the biotin/avidin system being
used.  The AP substrate may also be a little old - would this cause
background staining or just less colour production? Thankyou for your help.

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