Filling in without chewing back

Tom Bickle bickle at ubaclu.unibas.ch
Sun Jul 5 02:40:28 EST 1998


In article <359EA466.3850 at uidaho.edu>, Kajetan Groicher
<groi9663 at uidaho.edu> wrote:

> Hi-
> 
> Would Klenow Fragement (3'--5' exo-) work for this rxn?  It can be used
> at RT, and a claim is made that the 3'--5'exo activity is abolished.
> 
> Try New England Biolabs (no affiliation) www.neb.com.
> 
> Hope this helps-
> 
> Kajetan

Right, but not room temperature, use 6 degrees C (this turned out to be
the temp of our cold room at the time).



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