Smeared footprints

kathy gately kathy.gately at
Mon Jul 6 14:42:45 EST 1998

I'm using a footprinting method, adapted from that of Galas and Schmitz
and I'm getting alot of background smearing in the lanes containing the
footprints. Any ideas?
I was also wondering if anyone could recommend a good method/column for
probe purification? I'm digesting my probe with restrictions enzymes
that give a 3' end and a 5' end, then I 5' end-label with P32, I would
like a method of purification that would result in the probe being
eluted in a small final volume.
Thanks for any advice, kathy.

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