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Transfection of HepG2 cells

kathy gately kathy.gately at ucg.ie
Mon Jul 6 14:53:41 EST 1998

Has anybody transfected HepG2 cells with SV40-CAT or CMV enhanced
tk-CAT? I have done several transfections and I've used the CAT ELISA
kit by Boehringer-Mannheim to detect CAT activity, my readings are very
low for CAT, but are good for beta-gal, any ideas?
thanks for any advice, kathy

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