RT_PCR positive control

Malathi Hari mhari at expert.cc.purdue.edu
Mon Jul 6 12:21:20 EST 1998

I am trying to work out RT-PCR conditions to detect the expression of a 
small GTPase gene in various tissues and was wondering what positve control
to use.  I think I need to use Glyceraldehyde 3 phosphate dehydrogenase as
 a positve.  I was wondering if someone could point me in the right direction
and tell me where I can obtain primers or primer sequences for a postive 
Thanks in advance.


More information about the Methods mailing list