(none)

[Dr. N Kaushik]

Re: PCR based subtracted cDNA library.

There are some alternatives to do that:
1.DDRT-PCR
 This is quick and will be useful in your case.
2. PCR-Select subtraction kit provided by Clontech.
3.These references are quite useful: 

-BBRC 1997 230(2) 285
-Genomics 1996 38, 206-214
-Genomics 1997 46, 337-344
-Somat Cell Mol Genet 1997 23, 83-95.
Most interesting papers by Brady G et al
Curr Biol 1995 5, 909-922 and Method in Enzymol

Goo luck

Narendra