karenmk at cyllene.uwa.edu.au
Thu Jul 9 22:55:34 EST 1998
I am currently trying to determine the size of a DNA-binding protein using
UV-crosslinking. I bind by nuclear extract to a BrdU incorporated 32P
oligo, run reaction on a EMSA, then UV irradiate the gel, cut out the
complex, elute then run the elution on a SDS gel. The problem is I get
fuzzy bands, often many bands in one lane running as a streak or smear.
The complex runs low on EMSA, but gives several high MW proteins on SDS,
which runs on SDS with a smear. How can I get clearer bands on SDS. Any
help would be appreciated.
Thanks. Reply to email address please.
University of Western Australia
Email : karenmk at cyllene.uwa.edu.au
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