Regarding DEPC treatment
Philip Young, IWBT, University Stellenbosch
9369791 at SUN-AKAD2.SUN.AC.ZA
Thu Jul 9 21:09:38 EST 1998
Very normal - I find the smell quite soothing because I know the water/stock must be
RNase-free (I have however found that DEPC treatment is usually not necessary, as soon
as I spot degradation I am back on the DEPC and the degradation stops....coincidence?
Ambion (www.ambion.com) discuss the topic of DEPC-treatment for RNA work very
"Autoclaving DEPC-containing solutions should be of sufficient duration to rid the
solution of any smell.
FALSE. A faint EtOH smell may linger after autoclaving, but more commonly a
sweet, fruity smell is observed. This is caused by the EtOH byproduct combining with
trace carboxylic acid contaminates and forming volatile esters. It does not mean that trace
DEPC remains in the solution.
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