Sphingomyelinase and apoptosis

Prof. Luisa Dusonchet dusonc at unipa.it
Thu Jul 9 07:19:22 EST 1998

Dear collegues,
we need to evaluate the ability of exogenous acid sphingomyelinase in
inducing apoptosis in some tumor cell lines in vitro. For this purpose,
some authors (Zhang H et al. AACR 1998, abstract n. 3955) exposed the
cells to an acid medium (pH 6.0); however, in our experiments this pH
induces irreversible damages in our cell lines. Does anyone know how it
is possible to reproduce these experimental conditions without lethally
damaging the cells?

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