targeting cell lines

Ashok Aiyar aiyar at ebv.oncology.wisc.edu
Fri Jul 10 07:13:04 EST 1998


On Fri, 10 Jul 1998 11:05:13 +0200,
Tino Hochepied (tino at LMB.RUG.AC.BE) wrote:
>Is it possible to target a gene in cell lines (e.g. hek293T) by means of
>homologous recombination?

You can target genes by homologous recombination in cell lines, but 
bear in mind that most human cell lines are not diploid but are
aneuploid in their chromosomal content.  There are a few diploid
fibroblast lines available.  Another source of diploid immortalized
cells, are EBV-transformed lymphoid cell lines (LCLs).  You might be
better served using one of these diploid lines.

Ashok
-- 
Ashok Aiyar, Ph.D.
McArdle Laboratory for Cancer Research
aiyar at ebv.oncology.wisc.edu



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