bpmurray*STUFFER* at socrates.ucsf.edu
Fri Jul 10 16:53:12 EST 1998
In article <6o5dkv$eo2$1 at nnrp1.dejanews.com>, angstrom at my-dejanews.com wrote:
> Hello, all
> For the past year of two, I've been working with Costar polystyrene
> microtiter plates for ELISAs, but recently I've heard rumors about microtiter
> plates with higher binding capacities than these PS-only plates.
> Does anyone know if there are any of these "increased capacity" microtiter
> plates out there? If so please let me know.
It depends what you are after. There are specialised plates which
are surface treated to enhance binding of subsets of molecules
(eg. positively or negatively charged, biotinylated) but often a survey
of "normal" (and thus much cheaper) plates will reveal one that is
the best for you. Back in London we checked out over half a dozen
different plates (tissue culture. microtitre, and some "high binding"
plates) and there was a huge variation in performance. Our
particular winner was Dynatech's Immulon. Some of the "increased
capacity" plates certainly gave high signals but with greatly
increased backgrounds. If you *really* have to have high capacity
then I suggest you consider dot or slot blotting as an alternative
as this gives more quantitative loading (but is of course a real
pain if you have to run lots of samples).
I hope you find a good 'un,
[No commercial affiliation]
Bernard Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA
More information about the Methods