microtiter plates

Bernard Murray bpmurray*STUFFER* at socrates.ucsf.edu
Fri Jul 10 16:53:12 EST 1998


In article <6o5dkv$eo2$1 at nnrp1.dejanews.com>, angstrom at my-dejanews.com wrote:

> Hello, all
> For the past year of two, I've been working with Costar polystyrene
> microtiter plates for ELISAs, but recently I've heard rumors about microtiter
> plates with higher binding capacities than these PS-only plates.
> Does anyone know if there are any of these "increased capacity" microtiter
> plates out there? If so please let me know.
> Angstrom

It depends what you are after.  There are specialised plates which
are surface treated to enhance binding of subsets of molecules
(eg. positively or negatively charged, biotinylated) but often a survey
of "normal" (and thus much cheaper) plates will reveal one that is
the best for you.  Back in London we checked out over half a dozen
different plates (tissue culture. microtitre, and some "high binding"
plates) and there was a huge variation in performance.  Our
particular winner was Dynatech's Immulon.  Some of the "increased
capacity" plates certainly gave high signals but with greatly
increased backgrounds.  If you *really* have to have high capacity
then I suggest you consider dot or slot blotting as an alternative
as this gives more quantitative loading (but is of course a real
pain if you have to run lots of samples).
     I hope you find a good 'un,
          Bernard

[No commercial affiliation]
-- 
Bernard Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA



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