Acrylamide Gel Problem.
christenw at fnr.purdue.edu
Fri Jul 10 13:17:22 EST 1998
I agree with Mike, Rainex works really well. I was having an odd problem, my
gels were completely sticking to the upper plate (I had been using
Sigmacote). It wasn't a problem as long as the whole gel stuck, but I
switched to Rainex and now the gel stays were it should. You might want to
strip your lower plate to make sure it hasn't got any silicone on it that's
screwing things up. The folks at Sigma seemed to think it might be related
to the humidity levels (?). That's not something I have time to worry
Michael Black, Ph.D. wrote:
> Greg, instead of silane, just get hold of some RainX (the stuff for
> car windows). It should be available in any auto-parts store or
> general hardware store. It costs only a couple of bucks a bottle and
> is not nearly as nasty to your innards as silane and chloroform.
> I have also in the distant past used a methacrylite (sp?) glue to bond
> the gel to the front plate. The name of the product and protocol are
> in my office, but I can e-mail it to you later if interested.
> Cheers, Michael
> On Wed, 08 Jul 1998 13:11:49 +0100, Greg <g.dean at uea.ac.uk> wrote:
> >Just a simple problem, I am having problems of keeping my sequencing
> >gels (for footprinting) on the front plate when I remove the silicone
> >treated back plate and have been trying to find a company which sell an
> >'adhesive' (non-covalent) treatment for the front plate. I'm not even
> >sure of the name of the product. Any help, as always gratefully
> >Thanks in advance.
> >Dr. Greg Dean
> >Dept. of Biological Sciences
> >University of East Anglia
> >NR4 7TJ England
> >Tel.: 01603 593796
> >Fax.: 01603 592250
> Michael Black, Ph.D.
> Post-Doctoral Fellow
> Fisheries & Oceans, Canada
> mbblack at globetrotter.qc.ca
> * opinions expressed are, as far *
> * as I know, just my own. *
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