Regarding DEPC treatment

ELADER at AMBION.COM ELADER at AMBION.COM
Sun Jul 12 17:56:08 EST 1998


In article <9E88966FE7 at akad2.sun.ac.za>,
  9369791 at SUN-AKAD2.SUN.AC.ZA ("Philip Young, IWBT, University Stellenbosch")
wrote:
> Very normal - I find the smell quite soothing because I know the water/stock
must be
> RNase-free (I have however found that DEPC treatment is usually not necessary,
as soon
> as I spot degradation I am back on the DEPC and the degradation
stops....coincidence?
> Ambion (www.ambion.com) discuss the topic of DEPC-treatment for RNA work very
> thoroughly:
>     "Autoclaving DEPC-containing solutions should be of sufficient duration to
rid the
> solution of any smell.
>            FALSE. A faint EtOH smell may linger after autoclaving, but more
commonly a
> sweet, fruity smell is observed. This is caused by the EtOH byproduct
combining with
> trace carboxylic acid contaminates and forming volatile esters. It does not
mean that trace
> DEPC remains in the solution.
>

Ambion does double autoclave it's water.

Eric
elader at ambion.com

-----== Posted via Deja News, The Leader in Internet Discussion ==-----
http://www.dejanews.com/rg_mkgrp.xp   Create Your Own Free Member Forum



More information about the Methods mailing list