Non radioactive northerns
andrew.bettany at bbsrc.ac.uk
Mon Jul 13 02:45:48 EST 1998
We have been using the Boehringer DIG system using in vitro transcribed RNA
probes. We're very happy with the results with one exception: stripping
probes is pretty difficult! (even boiling 0.1% SDS doesn't always work!). We
are considering combining DIG-labelling with the Ambion Strip-EZ system -
unfortunately Ambion haven't tried this and can't tell us if it will work or
Dr. Andy Bettany
Institute of Grassland and Environmental Research
Philippe Lefebvre wrote in message
<126.96.36.199.19980708103547.0127d3c0 at biserte.lille.inserm.fr>...
>This is just for a quick survey of what you are currently using for non
>radioactive detection of RNA. Any comment on pros and cons of commercially
>available kits would be welcome!
>Thanks for your help on this!
>Faculte de Medecine Henri Warembourg
>1 place de verdun
>59045 Lille cedex
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