Sequencing G-C rich DNA

h.brown h.brown at microbiology.unimelb.edu.au
Tue Jul 14 05:01:42 EST 1998


Hi

Does anyone have a method for sequencing G-C rich cloned DNA?

The sequencing PCR conditions we are currently using for all sequencing
reactions regardless of nucleotide sequence is :

	96'C	10 sec
	50'C	5 secs
	60'C	4 mins

for 25 cycles.

Please let me know....

Hayley - The Comet

------------
Hayley Brown
Dept of Microbiology and Immunology
University of Melbourne
(03) 9344 7875



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