PCR

Rafael Maldonado rmaldonado at jamonua.es
Mon Jul 13 08:08:10 EST 1998


carin wrote:
> 
> >Hi there,
> >We want to put on sites for restriction enzymes on the ends of our gene
> 
> >of interest by PCR. Would anybody have a manual, a reference  or tips
> on
> >what to consider etc?

The most important thing is to choose one enzime able to cut close to an
endo of the DNA. In the New England Bioloabs catalog there is
information about this for some of the enzymes. 

-Rafa

-- 
Rafael Maldonado
Division of Genetics
University of Alicante
rmaldonado at jamonua.es 
(don´t use "jamon" to contact me)



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