Gentle Plasmid prep protocol needed

Michael Kuzyk icarus at uvic.ca
Mon Jul 13 13:46:02 EST 1998


If anyone has a protocol for purifying high copy # plasmid that yields
almost completely supercoiled plasmid please post it. I'm currently trying
to make a nested deletion library with ExoIII/S1 nuclease and am having
trouble with ExoIII digestion starting at nicks.

Thanks,
Mike



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