cell permeabilization

Richard V. Giles giles at liv.ac.uk
Tue Jul 14 09:04:21 EST 1998

U.DEligezer at DKFZ-Heidelberg.de wrote:

> HI ,
> Does anybody know how cells to permeabilize in cell culture.
> I try to enter my recombinant proteins into the cells.
> I would be very greatful for any help.

 You could try streptolysin O permeabilisation (detailed protocol on my
web site - methods section).

I have also tried pinosomal lysis (attempting to deliver
oligodeoxynucleotides), but had very little success

Richard V. Giles Ph.D.
School of Biological Sciences
University of Liverpool
Tel +44/151 794 4389
Fax +44/151 794 4349

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