Best way to store DNA?

Conrad R Fjetland fjetland at bcm.tmc.edu
Tue Jul 14 14:23:47 EST 1998


Granger L. Ridout (granger.ridout at murraystate.edu) wrote:
: Please forgive this possibly dumb question, but what is the best way to
: store (environmental samples) bacterial DNA after EtOH precipitation?  I
: am looking for long-term (6 months) as well as short-term (few days)
: storage.  Is it best to leave the DNA pellet dried and stored at -70C
: (or liquid nitrogen?), resuspended in deionized water or TE and frozen,
: or resuspended and stored at -20/0C?  Again, please forgive my
: ignorance.  And thanks!

: G.L.Ridout

The way we always stored our DNA samples was in TE  at 4 C.  I never 
freeze my DNA for fear of nicking during the thawing process (This is just
a
personal preference). DNA is such astable molecule, you could conceivably
leave it on your bench top and have no problems.



Conrad R. Fjetland




More information about the Methods mailing list