Best way to store DNA?
jladasky at pmgm.Stanford.EDU
Tue Jul 14 14:49:07 EST 1998
In article <6ogb83$qde at gazette.bcm.tmc.edu>,
Conrad R Fjetland <fjetland at bcm.tmc.edu> wrote:
>Granger L. Ridout (granger.ridout at murraystate.edu) wrote:
>: Please forgive this possibly dumb question, but what is the best way to
>: store (environmental samples) bacterial DNA after EtOH precipitation? I
>: am looking for long-term (6 months) as well as short-term (few days)
>: storage. Is it best to leave the DNA pellet dried and stored at -70C
>: (or liquid nitrogen?), resuspended in deionized water or TE and frozen,
>: or resuspended and stored at -20/0C? Again, please forgive my
>: ignorance. And thanks!
>The way we always stored our DNA samples was in TE at 4 C. I never
>freeze my DNA for fear of nicking during the thawing process (This is just
>a personal preference). DNA is such astable molecule, you could conceivably
>leave it on your bench top and have no problems.
Most DNA samples still contain residual nuclease activity, even af-
ter purification. It is probably wisest to store them at 4 degrees rather
than at room temperature.
Rainforest laid low.
"Wake up and smell the ozone,"
Says man with chainsaw. - John Ladasky
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