requeriment of DNA immunization vectors-01

[JUAN MORALES GRILLO]

Hi all:
Someone could tell me somethings about: a) presence of intron in the 
vector?; b) or sequence of Kosack in the vector in order to get good 
expression of the protein?; c) experiences on use of signal 
sequences.
I am waiting for help, juan  
Center for Genetic Engineering and Biotechnology.
Hepatitis C Department.
Vaccines Division.
PO Box 6162, ZP 10600
Cubanacan, Ciudad Habana, Cuba.
Fax: (53-7)218070 Phone: 53-7-218008/218446/218164
E-mail: Juan.Morales at cigb.edu.cu