purification plasmids for DNA immunization

JUAN MORALES GRILLO Juan.Morales at CIGB.EDU.CU
Tue Jul 14 12:41:20 EST 1998


Hi all net-members:
We are new, and we want to get a good protocolle for purification 
plasmid for DNA immunization, one for small scale and other for large 
scale using chromatografic methods.
We also want to know about requirements of the prep plasmid like: 
free of endotoxin??; supercoil??; presence of RNA?, proteins?
That's all for some minutes, juan 
Center for Genetic Engineering and Biotechnology.
Hepatitis C Department.
Vaccines Division.
PO Box 6162, ZP 10600
Cubanacan, Ciudad Habana, Cuba.
Fax: (53-7)218070 Phone: 53-7-218008/218446/218164
E-mail: Juan.Morales at cigb.edu.cu





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