cabe at stolaf.edu
Tue Jul 14 11:16:16 EST 1998
Two questions regarding DOP-PCR, which we are running for the first
time. We are amplifying genomic DNA from plants.
1. I was expecting a nice smear on my 1% agarose gel, much like RE
digested genomic DNA. I did get a smear of sorts, with most of the
amplified DNA ranging from from about 600 bp to 150 bp. It is not a
completely "even" smear - there appear to be some brighter bands. Does
this sound like complete success?
2. Does anyone have a convenient positive control for DOP-PCR?
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